Hao Jianxiong, Wu Tongjiao, Li Huiying, Wang Wei, Liu Haijie
College of Bio Science and Engineering, Hebei University of Science and Technology, No. 70 Yuhuadonglu, Shijiazhuang, Hebei 050018, PR China.
Shijiazhuang Academy of Agricultural and Forestry Science, No. 70 Shenglibeilu, Shijiazhuang, Hebei 050000, PR China.
Food Chem. 2016 Jun 15;201:87-93. doi: 10.1016/j.foodchem.2016.01.037. Epub 2016 Jan 11.
In the present study, the dual effects of slightly acidic electrolyzed water (SAEW) treatment on γ-aminobutyric acid (GABA) and rutin accumulation of germinated buckwheat were evaluated during germination. The results showed that SAEW treatment (pH 5.83, ACC of 20.3 mg/L) could promote the accumulation of GABA and rutin in germinated buckwheat. The GABA and rutin contents of SAEW-germinated buckwheat reached 143.20 and 739.9 mg/100 g respectively, which is significantly higher than those of control (P<0.05). Moreover, SAEW treatment could increase the activity of glutamic acid decarboxylase (GAD) and phenylalanine ammonialyase (PAL) and thus result in the GABA and rutin accumulation of germinated buckwheat. The results suggested that SAEW treatment could promote the rutin accumulation of germinated buckwheat by influencing phenylpropanoid secondary metabolic pathway instead of the inhibition of rutin degrading enzyme (RDE) activity. In addition, SAEW treatment had no adverse impact on the sprouts growth and could reduce the microbial populations of germinated buckwheat during germination.
在本研究中，评估了微酸性电解水（SAEW）处理对发芽荞麦在发芽过程中γ-氨基丁酸（GABA）和芦丁积累的双重影响。结果表明，SAEW处理（pH 5.83，ACC为20.3 mg/L）可促进发芽荞麦中GABA和芦丁的积累。SAEW发芽荞麦的GABA和芦丁含量分别达到143.20和739.9 mg/100 g，显著高于对照（P<0.05）。此外，SAEW处理可提高谷氨酸脱羧酶（GAD）和苯丙氨酸解氨酶（PAL）的活性，从而导致发芽荞麦中GABA和芦丁的积累。结果表明，SAEW处理可能通过影响苯丙烷类次生代谢途径而非抑制芦丁降解酶（RDE）活性来促进发芽荞麦中芦丁的积累。此外，SAEW处理对芽苗生长没有不利影响，并且可以在发芽过程中减少发芽荞麦的微生物数量。
