Beijing Advanced Innovation Center for Food Nutrition and Human Health , China Agricultural University , Beijing 100083 , China.
College of Food Science and Nutritional Engineering , China Agricultural University , Beijing 100083 , China.
J Agric Food Chem. 2019 Jan 16;67(2):606-614. doi: 10.1021/acs.jafc.8b04958. Epub 2019 Jan 8.
Changes in the content of bioactive phytochemicals in the broccoli sprouts subjected to different slightly acidic electrolyzed water (SAEW) treatments were investigated in the present study. The highest sulforaphane amount in broccoli sprouts treated with SAEW with an available chlorine concentration (ACC) of 50 mg/L was 11.49 mg/g in dry weight (DW), which increased by 61.2% compared to the control. SAEW treatment enhanced the sulforaphane content mainly by increasing the glucoraphanin (GRA) concentration due to the promotion of methionine metabolism and increased myrosinase activities. In addition, the relative anthocyanin contents of light-germinated broccoli under SAEW 50 treatment were 2.03 times that of broccoli sprouts with tap water treatment, and these contents were associated with an increase in phenylalanine ammonia lyase (PAL) activities and phenylalanine participation in biosynthesis. In summary, SAEW promotes metabolism to induce the accumulation of bioactive compounds in broccoli sprouts.
本研究探讨了不同酸性电解水(SAEW)处理对西兰花芽中生物活性植物化学物质含量的影响。经 50mg/L 有效氯浓度(ACC)SAEW 处理的西兰花芽中,萝卜硫素含量最高,达 11.49mg/g 干重(DW),比对照组增加了 61.2%。SAEW 处理主要通过增加葡萄糖异硫氰酸盐(GRA)浓度来提高萝卜硫素含量,这是由于促进了蛋氨酸代谢和增加了糜蛋白酶的活性。此外,SAEW50 处理的轻度发芽西兰花的相对花青素含量是自来水处理的西兰花芽的 2.03 倍,这与苯丙氨酸解氨酶(PAL)活性的增加以及苯丙氨酸参与生物合成有关。总之,SAEW 通过促进代谢来诱导西兰花芽中生物活性化合物的积累。