Graziani F, Pujol A, Nicoletti C, Dou S, Maresca M, Giardina T, Fons M, Perrier J
iSm2 UMR 7313, CNRS, Centrale Marseille, Aix Marseille Université, Marseille, France.
UP 2012.10.120.EGEAL, Institut Polytechnique, La Salle Beauvais, France.
J Appl Microbiol. 2016 May;120(5):1403-17. doi: 10.1111/jam.13095. Epub 2016 Apr 4.
The molecular cross-talk between commensal bacteria and the gut play an important role in the maintenance of the intestinal homeostasis and general health. Here, we studied the impact of a major Gram-positive anaerobic bacterium of the human gut microbiota, that is, Ruminococcus gnavus on the glycosylation pattern and the production of intestinal mucus by the goblet cells.
Our results showed that R. gnavus E1 specifically increases the expression and the glycosylation level of the intestinal glyco-conjugates by goblet cells in the colonic mucosa of mono-associated mice with R. gnavus E1 as well as in human HT29-MTX cells. Such an effect was mediated through induction of the level of mRNA encoding for the major intestinal gel-forming mucin such as MUC2 and various glycosyltransferase enzymes.
This study demonstrates for the first time that R. gnavus E1 possess the ability to modulate the glycosylation profile of the glyco-conjugate molecules and mucus in goblet cells.
Furthermore, we demonstrated that R. gnavus E1 modified specifically the glycosylation pattern and MUC2 expression by means of a small soluble factor of peptidic nature (<3 kDa) and heat stable in the HT29-MTX cell.
共生细菌与肠道之间的分子相互作用在维持肠道稳态和整体健康方面发挥着重要作用。在此，我们研究了人类肠道微生物群中一种主要的革兰氏阳性厌氧菌，即纤细瘤胃球菌对杯状细胞糖基化模式和肠道黏液产生的影响。
我们的结果表明，在以纤细瘤胃球菌E1单定殖的小鼠结肠黏膜以及人HT29 - MTX细胞中，纤细瘤胃球菌E1特异性地增加了杯状细胞对肠道糖缀合物的表达和糖基化水平。这种效应是通过诱导主要的肠道凝胶形成黏蛋白（如MUC2）以及各种糖基转移酶的mRNA水平来介导的。
本研究首次证明纤细瘤胃球菌E1具有调节杯状细胞中糖缀合物分子和黏液糖基化谱的能力。
此外，我们证明纤细瘤胃球菌E1通过一种小的肽性质（<3 kDa）且在HT29 - MTX细胞中热稳定的可溶性因子特异性地改变了糖基化模式和MUC2表达。
