Jing Chan Jer, Seman Idris Abu, Zakaria Latiffah
School of Biological Sciences, Universiti Sains Malaysia, 11800 USM, Pulau Pinang, Malaysia.
Malaysian Palm Oil Board, Biological Research Division, No 6 Persiaran Institusi, Bandar Baru Bangi, 43000 Kajang, Selangor, Malaysia.
Trop Life Sci Res. 2015 Dec;26(2):45-57.
Mating compatibility and restriction analyses of Internal Transcribed Spacer (ITS) regions were performed to determine the relations between Ganoderma boninense, the most common species associated with basal stem rot in oil palm and Ganoderma isolates from infected oil palm, two ornamental palms, sealing wax palm (Cyrtostachys renda) and MacArthur palm (Ptychosperma macarthurii), an isolate from coconut stump (Cocos nucifera), Ganoderma miniatocinctum, Ganoderma zonatum and Ganoderma tornatum. The results showed that G. boninense was compatible with Ganoderma isolates from oil palm, G. miniatocinctum and G. zonatum, Ganoderma isolates from sealing wax palm, MacArthur palm and coconut stump. G. boninense was not compatible with G. tornatum. Therefore, the results suggested that the G. boninense, G. miniatocinctum, G. zonatum, and Ganoderma isolates from oil palm, ornamental palms and coconut stump could represent the same biological species. In performing a restriction analysis of the ITS regions, variations were observed in which five haplotypes were generated from the restriction patterns. An unweighted pair-group method with arithmetic averages (UPGMA) cluster analysis showed that all the Ganoderma isolates were grouped into five primary groups, and the similarity values of the isolates ranged from 97% to 100%. Thus, a restriction analysis of the ITS regions showed that G. boninense and the Ganoderma isolates from other palm hosts were closely related. On the basis of the mating compatibility test and the restriction analysis of the ITS regions performed in this study, a diverse group of Ganoderma species from oil palm and other palm hosts are closely related, except for G. tornatum and Ganoderma isolates from tea and rubber.
对内部转录间隔区（ITS）进行交配相容性和限制性分析，以确定与油棕基部茎腐病相关的最常见物种——邦氏灵芝，以及来自感染油棕、两种观赏棕榈（红刺露兜树和麦氏皱籽椰）、椰子树桩分离株、小带灵芝、环纹灵芝和撕裂灵芝的灵芝分离株之间的关系。结果表明，邦氏灵芝与来自油棕、小带灵芝和环纹灵芝的灵芝分离株、来自红刺露兜树、麦氏皱籽椰和椰子树桩的灵芝分离株交配相容。邦氏灵芝与撕裂灵芝不相容。因此，结果表明，邦氏灵芝、小带灵芝、环纹灵芝以及来自油棕、观赏棕榈和椰子树桩的灵芝分离株可能代表同一生物物种。在对ITS区域进行限制性分析时，观察到了变异，从限制性图谱中产生了五种单倍型。算术平均数的非加权配对组方法（UPGMA）聚类分析表明，所有灵芝分离株被分为五个主要组，分离株的相似性值在97%至100%之间。因此，对ITS区域的限制性分析表明，邦氏灵芝与来自其他棕榈寄主的灵芝分离株密切相关。基于本研究中进行的交配相容性测试和ITS区域的限制性分析，除了来自茶树和橡胶树的撕裂灵芝和灵芝分离株外，来自油棕和其他棕榈寄主的不同灵芝物种密切相关。
