Simon Hannah, Hoffmann Grete, Hübner Florian, Humpf Hans-Ulrich, Karst Uwe
Institute of Inorganic and Analytical Chemistry, Westfälische Wilhelms-Universität Münster, Corrensstraße 30, 48149, Münster, Germany.
Institute of Food Chemistry, Westfälische Wilhelms-Universität Münster, Corrensstr. 45, 48149, Münster, Germany.
Anal Bioanal Chem. 2016 Apr;408(10):2471-83. doi: 10.1007/s00216-016-9344-z. Epub 2016 Feb 11.
Mycotoxins are secondary plant metabolites that have been found to cause severe diseases in humans and livestock. Exposure can take place on a daily basis since mycotoxins can be found not only in food, animal food, and dietary supplements but also in materials used in buildings. For this work, the Alternaria toxins alternariol (AOH) and alternariol methyl ether (AME) are chosen as representatives for this relevant compound class and are investigated regarding their oxidative phase I metabolism using a combination of electrochemical (EC) oxidation and high-resolution electrospray ionization mass spectrometry (HR-ESI-MS). This previously established method has been proven to be a valuable tool for the electrochemical simulation of certain phase I metabolic reactions. A comparison of the electrochemically generated products with those formed during microsomal incubation demonstrates the potential of the method for the successful prediction of the main phase I metabolic reactions of mycotoxins. It can thus find use as a supportive method in the elucidation of the metabolic pathways of various mycotoxins.
霉菌毒素是植物的次生代谢产物，已被发现可导致人类和牲畜患上严重疾病。由于霉菌毒素不仅存在于食品、动物饲料和膳食补充剂中，还存在于建筑材料中，因此人们每天都可能接触到它们。在这项研究中，选择链格孢毒素交替菌素（AOH）和交替菌素甲醚（AME）作为这类相关化合物的代表，并结合电化学（EC）氧化和高分辨率电喷雾电离质谱（HR-ESI-MS）研究它们的氧化I相代谢。这种先前建立的方法已被证明是电化学模拟某些I相代谢反应的宝贵工具。将电化学生成的产物与微粒体孵育过程中形成的产物进行比较，证明了该方法成功预测霉菌毒素主要I相代谢反应的潜力。因此，它可以作为一种辅助方法，用于阐明各种霉菌毒素的代谢途径。
