Lamattina L, Weil J H, Grienenberger J M
Institut de Biologie Moléculaire des Plantes du CNRS, Université Louis Pasteur, Strasbourg, France.
FEBS Lett. 1989 Nov 20;258(1):79-83. doi: 10.1016/0014-5793(89)81620-5.
Comparison between the sequence of the gene coding for the wheat mitochondrial NADH dehydrogenase subunit IV (nad4) and the cDNA sequence obtained by reverse transcription, using total wheat mtRNA as template, has shown the presence of a uridine residue, not encoded by the genomic sequence, at the exon2-exon3 junction of the spliced transcript. This U creates a non-encoded CUG leucine codon which is essential for maintaining the reading frame, as shown by the conservation of the amino acid sequence of the NAD4 protein in various species. The addition of a U or the specific post-transcriptional conversion of a C to a U could explain this phenomenon.
对小麦线粒体烟酰胺腺嘌呤二核苷酸脱氢酶亚基IV（nad4）编码基因的序列与以小麦线粒体总RNA为模板通过逆转录获得的cDNA序列进行比较，结果显示，在剪接转录本的外显子2-外显子3交界处存在一个基因组序列未编码的尿苷残基。这个U产生了一个非编码的CUG亮氨酸密码子，如不同物种中NAD4蛋白氨基酸序列的保守性所示，该密码子对于维持阅读框至关重要。添加一个U或C到U的特定转录后转化可以解释这一现象。
