Haendler B, Keller R, Hiestand P C, Kocher H P, Wegmann G, Movva N R
Preclinical Research, Sandoz Ltd., Basle, Switzerland.
Gene. 1989 Nov 15;83(1):39-46. doi: 10.1016/0378-1119(89)90401-0.
Cyclophilin (CPH) has been isolated from the yeast Saccharomyces cerevisiae, purified to homogeneity and partially sequenced. Oligodeoxyribonucleotides deduced from this sequence were used to isolate the corresponding cDNA and gene. An open reading frame coding for a 162-amino acid (aa) protein with a calculated Mr of 17,392, was deduced from the nucleotide sequence. Comparison between yeast and human CPH shows a very high overall sequence conservation (65% aa homology). The binding of yeast CPH to cyclosporin A is identical to that of human and bovine CPH. Unlike in Neurospora crassa, a mitochondrial form of CPH could not be detected in yeast. Southern-blot analysis of yeast DNA shows that only one CPH-related sequence is present per haploid genome, whereas at least 20 genes or pseudogenes were detected in the human and rat genome. Purified yeast CPH exhibits peptidyl-prolyl cis-trans isomerase activity, albeit to a far lesser extent than the mammalian protein.
亲环蛋白（CPH）已从酿酒酵母中分离出来，纯化至同质并进行了部分测序。根据该序列推导的寡脱氧核糖核苷酸用于分离相应的cDNA和基因。从核苷酸序列推导得出一个开放阅读框，编码一种162个氨基酸（aa）的蛋白质，计算得出的分子量为17392。酵母和人CPH之间的比较显示出非常高的整体序列保守性（65%氨基酸同源性）。酵母CPH与环孢素A的结合与人及牛CPH相同。与粗糙脉孢菌不同，在酵母中未检测到线粒体形式的CPH。酵母DNA的Southern杂交分析表明，每个单倍体基因组中仅存在一个与CPH相关的序列，而在人和大鼠基因组中检测到至少20个基因或假基因。纯化的酵母CPH表现出肽基脯氨酰顺反异构酶活性，尽管程度远低于哺乳动物蛋白。
