Valta Maija P, Zhao Hongjuan, Saar Matthias, Tuomela Johanna, Nolley Rosalie, Linxweiler Johannes, Sandholm Jouko, Lehtimäki Jaakko, Härkönen Pirkko, Coleman Ilsa, Nelson Peter S, Corey Eva, Peehl Donna M
Department of Urology, Stanford University School of Medicine, Stanford, CA, 94305, USA.
Division of Medicine, Turku University Hospital and University of Turku, Turku, Finland.
Clin Exp Metastasis. 2016 Apr;33(4):325-37. doi: 10.1007/s10585-016-9781-2. Epub 2016 Feb 12.
LuCaP serially transplantable patient-derived xenografts (PDXs) are valuable preclinical models of locally advanced or metastatic prostate cancer. Using spheroid culture methodology, we recently established cell lines from several LuCaP PDXs. Here, we characterized in depth the features of xenografts derived from LuCaP 136 spheroid cultures and found faithful retention of the phenotype of the original PDX. In vitro culture enabled luciferase transfection into LuCaP 136 spheroids, facilitating in vivo imaging. We showed that LuCaP 136 spheroids formed intratibial, orthotopic, and subcutaneous tumors when re-introduced into mice. Intratibial tumors responded to castration and were highly osteosclerotic. LuCaP 136 is a realistic in vitro-in vivo preclinical model of a subtype of bone metastatic prostate cancer.
LuCaP系列可连续移植的患者来源异种移植瘤(PDXs)是局部晚期或转移性前列腺癌有价值的临床前模型。利用球体培养方法,我们最近从几个LuCaP PDXs建立了细胞系。在此,我们深入表征了源自LuCaP 136球体培养物的异种移植瘤的特征,发现其忠实保留了原始PDX的表型。体外培养使得荧光素酶能够转染到LuCaP 136球体中,便于体内成像。我们表明,将LuCaP 136球体重新植入小鼠体内时会形成胫骨内、原位和皮下肿瘤。胫骨内肿瘤对去势有反应,且高度骨硬化。LuCaP 136是骨转移性前列腺癌一种亚型的真实体外-体内临床前模型。