[GABAB受体表达水平对诱导癫痫大鼠模型认知功能障碍及Arc/Arg3.1表达的影响]

[Effects of GABAB receptor expression level on cognitive impairment and Arc/Arg3.1 expression in induced epileptic rats model].

作者信息

Lan Yanping, Sun Tao, Zhang Chun, Yuan Congcong, Yang Zheng, Wang Feng

机构信息

Department of Neurosurgery, General Hospital of Ningxia Medical University, Ningxia Key Laboratory of Cerebrocranial Diseases, the Incubation Base of National Key Laboratory, Ningxia Medical University, Yinchuan 750004, China.

出版信息

Zhonghua Yi Xue Za Zhi. 2016 Feb 2;96(5):380-3. doi: 10.3760/cma.j.issn.0376-2491.2016.05.014.

DOI:10.3760/cma.j.issn.0376-2491.2016.05.014

PMID:26875720

Abstract

OBJECTIVE

To investigate the effects of GABAB receptor on cognitive impairment by using pilocarpine induced kindled rats model and also to check early gene (Arc/Arg3.1) expression.

METHODS

Pilocarpine induced kindled rats were divided into four groups (Group normal, Baclofen, CGP and Kindled) randomly, and every group included 20 rats.We checked their cognitive impairment by using passive avoidance test and water maze test.The expression of GABAB receptor (GB1, GB2) and Arc/Arg3.1 was tested by immunohistochemical staining, RT-PCR and Western blot.

RESULTS

Passive avoidance test showed four Group rats shuttle times were 6.8±0.6, 1.2±0.2, 5.4±0.5, 3.6±0.3, incubation period were 26.1±3.9, 152.2±12.9, 65.8±7.0, 91.2±9.1, and water maze test had the same trend, with values in epilepsy groups significantly lower than the normal group of rats, which meant cognitive dysfunction.The above results also showed Baclofen further inhibited the learning and memory ability of the rats and CGP35348 promoted the learning and memory ability.The results of the Arc/Arg3.1 and GB1, GB2 level detection showed that epilepsy groups had significantly higher expression levels of Arc/Arg3.1 and GB1, GB2 than the normal group.Comparison among epilepsy groups showed that Baclofen group expressed lower levels of Arc/Arg3.1 and expressed higher levels of GB1, GB2, however CGP35348 group expressed higher levels of Arc/Arg3.1 and expressed lower levels of GB1, GB2.

CONCLUSIONS

GABAB receptor can affect the ability of spatial learning and memory of epileptic rats by regulating Arc/Arg3.1.

摘要

目的

采用毛果芸香碱诱导点燃大鼠模型，研究GABAB受体对认知障碍的影响，并检测早期基因（Arc/Arg3.1）的表达。

方法

将毛果芸香碱诱导点燃的大鼠随机分为四组（正常组、巴氯芬组、CGP组和点燃组），每组20只大鼠。通过被动回避试验和水迷宫试验检测其认知障碍。采用免疫组织化学染色、RT-PCR和Western blot检测GABAB受体（GB1、GB2）和Arc/Arg3.1的表达。

结果

被动回避试验显示，四组大鼠的穿梭次数分别为6.8±0.6、1.2±0.2、5.4±0.5、3.6±0.3，潜伏期分别为26.1±3.9、152.2±12.9、65.8±7.0、91.2±9.1，水迷宫试验结果趋势相同，癫痫组的值显著低于正常组大鼠，这意味着存在认知功能障碍。上述结果还表明，巴氯芬进一步抑制了大鼠的学习和记忆能力，而CGP35348则促进了学习和记忆能力。Arc/Arg3.1以及GB1、GB2水平检测结果显示，癫痫组Arc/Arg3.1以及GB1、GB2的表达水平显著高于正常组。癫痫组之间比较显示，巴氯芬组Arc/Arg3.1表达水平较低，GB1、GB2表达水平较高，而CGP35348组Arc/Arg3.1表达水平较高，GB1、GB2表达水平较低。