Ding Yongbo, Zheng Jiong, Xia Xuejuan, Ren Tingyuan, Kan Jianquan
College of Food Science, Southwest University, Chongqing 400715, China; Laboratory of Quality & Safety Risk Assessment for Agro-products on Storage and Preservation (Chongqing), Ministry of Agriculture, Chongqing 400715, China.
College of Food Science, Southwest University, Chongqing 400715, China; Laboratory of Quality & Safety Risk Assessment for Agro-products on Storage and Preservation (Chongqing), Ministry of Agriculture, Chongqing 400715, China.
Carbohydr Polym. 2016 May 5;141:151-9. doi: 10.1016/j.carbpol.2016.01.008. Epub 2016 Jan 7.
This study aimed to assess the properties of resistant starch type IV (chemically modified starch, RS4) prepared from a new and convenient synthesis route by using ultrasonication combined with water-in-oil miniemulsion cross-linking technique. A three-factor Box-Behnken design and optimization was used to minimize particle size through the developed RS4 nanoparticles. The predicted minimized Z-Avel (576.1nm) under the optimum conditions of the process variables (ultrasonic power, 214.57W; sonication time, 114.73min; and oil/water ratio, 10.54:1) was very close to the experimental value (651.0nm) determined in a batch experiment. After preparing the RS4 nanoparticles, morphological, physical, chemical, and functional properties were assessed. Results revealed that RS4 nanoparticle size reached about 600nm. Scanning electron microscopy images showed that ultrasonication induced notches and grooves on the surface. Under polarized light, the polarized cross was impaired. X-ray diffraction results revealed that the crystalline structure was disrupted. Smaller or no endotherms were exhibited in DSC analysis. In the FTIR graph, new peaks at 1532.91 and 1451.50cm(-1) were observed, and pasting properties were reduced. Amylose content, solubility, and SP increased, but RS content decreased. Anti-digestibility remained after ultrasonication. The prepared RS4 nanoparticles could be extensively used in biomedical applications and in the development of new medical materials.
本研究旨在评估通过超声处理结合油包水微乳液交联技术，从一种新的便捷合成路线制备的IV型抗性淀粉（化学改性淀粉，RS4）的性质。采用三因素Box-Behnken设计和优化方法，通过制备的RS4纳米颗粒使粒径最小化。在工艺变量（超声功率214.57W、超声处理时间114.73分钟、油/水比10.54:1）的最佳条件下，预测的最小Z-平均粒径（576.1nm）与批次实验中测定的实验值（651.0nm）非常接近。制备RS4纳米颗粒后，对其形态、物理、化学和功能性质进行了评估。结果显示RS4纳米颗粒尺寸达到约600nm。扫描电子显微镜图像表明，超声处理在表面诱导出缺口和凹槽。在偏振光下，偏振十字受到破坏。X射线衍射结果表明晶体结构被破坏。差示扫描量热分析显示较小的吸热峰或无吸热峰。在傅里叶变换红外光谱图中，观察到在1532.91和1451.50cm⁻¹处出现新峰，且糊化性质降低。直链淀粉含量、溶解度和膨胀势增加，但抗性淀粉含量降低。超声处理后仍保留抗消化性。制备的RS4纳米颗粒可广泛应用于生物医学领域以及新型医用材料的开发。
