首次在绵羊中对斯氏副裸头绦虫核糖体DNA第一内转录间隔区进行分子特征分析。

Molecular characterization of the first internal transcribed spacer of rDNA of Parabronema skrjabini for the first time in sheep.

作者信息

Hasheminasab Seyed Sajjad

机构信息

Department of Parasitology, Faculty of Veterinary Medicine, University of Tehran, Qareeb St., Azadi Ave., 1419963111 Tehran, Iran; E-mail:

出版信息

Ann Parasitol. 2015;61(4):241-6. doi: 10.17420/ap6104.13.

DOI:10.17420/ap6104.13

PMID:26878620

Abstract

Parabronema skrjabini is a spirurid nematode of the family Habronematidae that lives in the abomasum of ruminants such as sheep and goats. The purpose of this study was to investigate the molecular aspects of Parabronema skrjabini in sheep. The worms were collected from sheep in Sanandaj (west of Iran). The first internal transcribed spacer (ITS) of ribosomal DNA (rDNA) nucleotide fragments of Parabronema skrjabini were amplified by polymerase chain reaction (PCR) using two pairs of specific primers (Para-Ir-R and Para-Ir-F). ITS1 homology in the sequence of this study was 69% compared with the sequence data in GenBank. To our knowledge, this is the first study in the world exploring the genetic diversity of P. skrjabini in sheep based on ITS1.

摘要

斯氏副柔线虫是柔线虫科的一种旋尾线虫，寄生于绵羊和山羊等反刍动物的皱胃中。本研究旨在探讨绵羊体内斯氏副柔线虫的分子特征。这些线虫采自伊朗西部桑纳杰的绵羊。使用两对特异性引物（Para-Ir-R和Para-Ir-F）通过聚合酶链反应（PCR）扩增斯氏副柔线虫核糖体DNA（rDNA）核苷酸片段的第一个内部转录间隔区（ITS）。本研究序列中的ITS1与GenBank中的序列数据相比同源性为69%。据我们所知，这是世界上首次基于ITS1探索绵羊体内斯氏副柔线虫遗传多样性的研究。