MOE Key Laboratory of Aquatic Product Safety/KLGHEI of Environment and Energy Chemistry, School of Chemistry and Chemical Engineering, Sun Yat-sen University, Guangzhou 510275, China.
MOE Key Laboratory of Aquatic Product Safety/KLGHEI of Environment and Energy Chemistry, School of Chemistry and Chemical Engineering, Sun Yat-sen University, Guangzhou 510275, China.
Sci Total Environ. 2016 Apr 15;550:1134-1140. doi: 10.1016/j.scitotenv.2015.11.108. Epub 2016 Feb 12.
In vivo solid-phase microextraction (SPME) sampling method coupled with gas chromatography-mass spectrometry (GC-MS) analysis was employed to trace the uptake and elimination of organophosphorus pesticides (OPPs) in two kinds of edible plants, cabbage (Brassica parachinensis) and aloe (Barbadensis). The metabolism of fenthion in aloe was also investigated by the liquid chromatography tandem mass spectrometry analysis (LC-MS/MS) to understand the fate of OPPs in living plants better. Transpiration stream concentration factor (TSCF) and depuration rate constants of the OPPs in living plants were obtained therein. The health risk of the OPPs treated aloe was estimated by the maximum residue limit (MRL) approach, and it revealed that the OPPs were rather safe for their fast degradable property. However, peak concentration of fenthion-sulfoxide was found to exceed the MRL and was higher than that of the parent fenthion, which indicated the potential risk of pesticide metabolites. This study highlighted the application of in vivo SPME for contaminant tracing in different living edible plants. The in vivo tracing method is very convenient and can provide more data to evaluate the risk of different pesticides, which are very important for the safety of agriculture production.
采用体内固相微萃取 (SPME) 采样方法结合气相色谱-质谱联用 (GC-MS) 分析技术，追踪两种食用植物（白菜和芦荟）中有机磷农药 (OPPs) 的吸收和消除情况。通过液相色谱串联质谱分析 (LC-MS/MS) 还研究了在芦荟中芬硫磷的代谢情况，以更好地了解 OPPs 在活体植物中的命运。获得了活体植物中 OPPs 的蒸腾流浓度因子 (TSCF) 和净化率常数。通过最大残留限量 (MRL) 方法评估了处理过的芦荟中 OPPs 的健康风险，结果表明，由于其快速降解特性，这些 OPPs 相当安全。然而，发现芬硫磷砜的峰值浓度超过了 MRL，且高于母体芬硫磷，这表明代谢物农药具有潜在风险。本研究强调了体内 SPME 在不同食用活体植物中污染物追踪的应用。体内追踪方法非常方便，可以提供更多数据来评估不同农药的风险，这对农业生产的安全非常重要。
