State Key Lab of Food Science and Technology, Jiangnan University, Wuxi, JiangSu 214122, People's Republic of China.
Cereals & Oils Nutrition Research Group, Academy of Science & Technology of State Administration of Grain, Beijing 100037, People's Republic of China.
Nanoscale. 2016 Mar 7;8(9):5245-53. doi: 10.1039/c5nr09171c.
A semi-quantitative and quantitative multi-immunochromatographic (ICA) strip detection assay was developed for the simultaneous detection of twenty types of mycotoxins from five classes, including zearalenones (ZEAs), deoxynivalenols (DONs), T-2 toxins (T-2s), aflatoxins (AFs), and fumonisins (FBs), in cereal food samples. Sensitive and specific monoclonal antibodies were selected for this assay. The semi-quantitative results were obtained within 20 min by the naked eye, with visual limits of detection for ZEAs, DONs, T-2s, AFs and FBs of 0.1-0.5, 2.5-250, 0.5-1, 0.25-1 and 2.5-10 μg kg(-1), and cut-off values of 0.25-1, 5-500, 1-10, 0.5-2.5 and 5-25 μg kg(-1), respectively. The quantitative results were obtained using a hand-held strip scan reader, with the calculated limits of detection for ZEAs, DONs, T-2s, AFs and FBs of 0.04-0.17, 0.06-49, 0.15-0.22, 0.056-0.49 and 0.53-1.05 μg kg(-1), respectively. The analytical results of spiked samples were in accordance with the accurate content in the simultaneous detection analysis. This newly developed ICA strip assay is suitable for the on-site detection and rapid initial screening of mycotoxins in cereal samples, facilitating both semi-quantitative and quantitative determination.
建立了一种半定量和定量多免疫层析（ICA）条带检测分析方法，用于同时检测谷物食品样品中五类二十种真菌毒素，包括玉米赤霉烯酮（ZENs）、脱氧雪腐镰刀菌烯醇（DONs）、T-2 毒素（T-2s）、黄曲霉毒素（AFs）和伏马菌素（FBs）。该分析方法选用了敏感且特异的单克隆抗体。半定量结果可在 20 min 内通过肉眼获得，ZENs、DONs、T-2s、AFs 和 FBs 的视觉检测限为 0.1-0.5、2.5-250、0.5-1、0.25-1 和 2.5-10 μg kg(-1)，截止值为 0.25-1、5-500、1-10、0.5-2.5 和 5-25 μg kg(-1)。使用手持式条带扫描读取器可获得定量结果，ZENs、DONs、T-2s、AFs 和 FBs 的计算检测限分别为 0.04-0.17、0.06-49、0.15-0.22、0.056-0.49 和 0.53-1.05 μg kg(-1)。加标样品的分析结果与同时检测分析的准确含量相符。新开发的 ICA 条带检测分析方法适用于谷物样品中真菌毒素的现场检测和快速初步筛选，可进行半定量和定量测定。
