Lianidou Evi S
Analysis of Circulating Tumor Cells Lab, Lab of Analytical Chemistry, Department of Chemistry, University of Athens, 15771, Greece.
Mol Oncol. 2016 Mar;10(3):431-42. doi: 10.1016/j.molonc.2016.01.011. Epub 2016 Feb 5.
A variety of molecular assays have been developed for CTCs detection and molecular characterization. Molecular assays are based on the nucleic acid analysis in CTCs and are based on total RNA isolation and subsequent mRNA quantification of specific genes, or isolation of genomic DNA that can be for DNA methylation studies and mutation analysis. This review is mainly focused on gene expression and methylation studies in CTCs in various types of cancer.
已经开发出多种用于循环肿瘤细胞(CTCs)检测和分子特征分析的分子检测方法。分子检测方法基于对CTCs中的核酸分析,包括基于总RNA分离及随后对特定基因的mRNA定量分析,或者分离可用于DNA甲基化研究和突变分析的基因组DNA。本综述主要聚焦于各类癌症中CTCs的基因表达和甲基化研究。