采用CellSearch方法检测和鉴定循环肿瘤细胞

Detection and Characterization of Circulating Tumor Cells by the CellSearch Approach.

作者信息

Coumans Frank, Terstappen Leon

机构信息

Department of Medical Cell BioPhysics, MIRA Research Institute, Faculty of Science and Technology, University of Twente, Drienerlolaan 5, 217, Enschede, 7500 AE, The Netherlands.

出版信息

Methods Mol Biol. 2015;1347:263-78. doi: 10.1007/978-1-4939-2990-0_18.

DOI:10.1007/978-1-4939-2990-0_18

PMID:26374323

Abstract

Cancer metastasis occurs when cells shed from a primary or metastatic tumor, enter the circulation, and begin to grow in distant locations of the body. With current techniques it is possible to measure the presence of a few circulating tumor cells (CTC) in a blood sample. Detection of even the presence of a very small number (one or more) of these CTC in a 7.5 mL blood sample with the CellSearch system is associated with a significant decrease in survival of patients with metastatic carcinomas. The techniques and definitions used for the detection and enumeration of CTC with the CellSearch system were validated in series of preclinical and prospective multicenter studies. After enumeration of the CTC, the cells can be isolated from the cartridge for the purpose of downstream single-cell analysis. In this chapter, we will describe in detail the sample acquisition, sample preparation, data acquisition, and assignment of CTC used in the CellSearch system.

摘要

当细胞从原发性或转移性肿瘤脱落，进入循环系统，并开始在身体的远处部位生长时，癌症转移就会发生。利用当前技术，能够在血液样本中检测到少量循环肿瘤细胞（CTC）。使用CellSearch系统在7.5毫升血液样本中检测到即使极少量（一个或多个）的这些CTC，也与转移性癌患者的生存率显著降低相关。用于通过CellSearch系统检测和计数CTC的技术及定义，在一系列临床前和前瞻性多中心研究中得到了验证。在对CTC进行计数后，可从试剂盒中分离出细胞，用于下游的单细胞分析。在本章中，我们将详细描述CellSearch系统中使用的样本采集、样本制备、数据采集以及CTC的识别。