Qiu Xuehong, Wu Chunyan, Cao Li, Ehlers Ralf-Udo, Han Richou
Guangdong Entomological Institute, Public Laboratory of Wild Animal Conservation and Utilization, Key Laboratory of Integrated Pest Management in Agriculture, Guangzhou 510260, China;
E-nema GmbH, Klausdorfer Str. 28-36, Schwentinental 24223, Germany
FEMS Microbiol Lett. 2016 Mar;363(6). doi: 10.1093/femsle/fnw035. Epub 2016 Feb 15.
Photorhabdus (Enterobacteriaceae) bacteria are pathogenic to insects and mutualistic with entomopathogenic Heterorhabditis nematodes. Photorhabdus luminescens subsp. akhurstii LN2, associated with Heterorhabditis indica LN2, shows nematicidal activity against H. bacteriophora H06 infective juveniles (IJs). In the present study, an rpoS mutant of P. luminescens LN2 was generated through allelic exchange to examine the effects of rpoS deletion on the nematicidal activity and nematode development. The results showed that P. luminescens LN2 required rpoS for nematicidal activity against H06 nematodes, normal IJ recovery and development of H. indica LN2, however, not for the bacterial colonization in LN2 and H06 IJs. This provides cues for further understanding the role of rpoS in the mutualistic association between entomopathogenic nematodes and their symbionts.
发光杆菌属（肠杆菌科）细菌对昆虫具有致病性，并与昆虫病原性异小杆线虫互利共生。与印巴异小杆线虫LN2相关的阿氏发光杆菌亚种LN2，对嗜菌异小杆线虫H06感染性幼虫（IJs）表现出杀线虫活性。在本研究中，通过等位基因交换产生了发光杆菌LN2的rpoS突变体，以研究rpoS缺失对杀线虫活性和线虫发育的影响。结果表明，发光杆菌LN2对H06线虫的杀线虫活性、印巴异小杆线虫LN2正常IJ恢复和发育需要rpoS，但在LN2和H06 IJs中细菌定殖不需要rpoS。这为进一步了解rpoS在昆虫病原线虫及其共生体之间互利共生关系中的作用提供了线索。
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