对参与发光杆菌LN2种间杀线虫活性的一个新基因的鉴定与表征
Identification and characterization of a novel gene involved in the trans-specific nematicidal activity of Photorhabdus luminescens LN2.
作者信息
Qiu Xuehong, Han Richou, Yan Xun, Liu Mingxing, Cao Li, Yoshiga Toyoshi, Kondo Eizo
机构信息
Sun Yat-sen University, Guangzhou, China.
出版信息
Appl Environ Microbiol. 2009 Jun;75(12):4221-3. doi: 10.1128/AEM.02967-08. Epub 2009 Apr 17.
Abstract
Photorhabdus luminescens subsp. akhurstii LN2 from Heterorhabditis indica LN2 showed nematicidal activity against axenic Heterorhabditis bacteriophora H06 infective juveniles (IJs). Transposon mutagenesis identified an LN2 mutant that supports the growth of H06 nematodes. Tn5 disrupted the namA gene, encoding a novel 364-residue protein and involving the nematicidal activity. The green fluorescent protein-labeled namA mutant was unable to colonize the intestines of H06 IJs.
摘要
来自印度异小杆线虫LN2的发光杆菌阿克赫斯特亚种LN2对无菌的嗜菌异小杆线虫H06感染性幼虫（IJs）表现出杀线虫活性。转座子诱变鉴定出一个支持H06线虫生长的LN2突变体。Tn5破坏了namA基因，该基因编码一种新的364个氨基酸残基的蛋白质，并与杀线虫活性有关。绿色荧光蛋白标记的namA突变体无法定殖在H06 IJs的肠道中。
相似文献
1
Identification and characterization of a novel gene involved in the trans-specific nematicidal activity of Photorhabdus luminescens LN2.对参与发光杆菌LN2种间杀线虫活性的一个新基因的鉴定与表征
Appl Environ Microbiol. 2009 Jun;75(12):4221-3. doi: 10.1128/AEM.02967-08. Epub 2009 Apr 17.
2
Genetic and proteomic characterization of rpoB mutations and their effect on nematicidal activity in Photorhabdus luminescens LN2.rpoB 基因突变的遗传和蛋白质组学特征及其对发光杆菌 LN2 杀线虫活性的影响。
PLoS One. 2012;7(8):e43114. doi: 10.1371/journal.pone.0043114. Epub 2012 Aug 17.
3
Photorhabdus luminescens LN2 requires rpoS for nematicidal activity and nematode development.发光光杆状菌LN2的杀线虫活性和线虫发育需要rpoS。
FEMS Microbiol Lett. 2016 Mar;363(6). doi: 10.1093/femsle/fnw035. Epub 2016 Feb 15.
4
Draft Genome Sequence and Annotation of the Entomopathogenic Bacterium Photorhabdus luminescens LN2, Which Shows Nematicidal Activity against Heterorhabditis bacteriophora H06 Nematodes.对发光光杆状菌LN2的基因组序列草案及注释，该菌株对嗜线虫致病杆菌H06线虫具有杀线虫活性。
Genome Announc. 2014 Dec 11;2(6):e01268-14. doi: 10.1128/genomeA.01268-14.
5
Photorhabdus luminescens subsp. noenieputensis subsp. nov., a symbiotic bacterium associated with a novel Heterorhabditis species related to Heterorhabditis indica.发光杆菌亚种诺伊氏亚种，一种与新型异小杆线虫相关的共生细菌，该线虫与印度异小杆线虫有关。
Int J Syst Evol Microbiol. 2013 May;63(Pt 5):1853-1858. doi: 10.1099/ijs.0.044388-0. Epub 2012 Sep 14.
6
Polyphasic classification of the genus Photorhabdus and proposal of new taxa: P. luminescens subsp. luminescens subsp. nov., P. luminescens subsp. akhurstii subsp. nov., P. luminescens subsp. laumondii subsp. nov., P. temperata sp. nov., P. temperata subsp. temperata subsp. nov. and P. asymbiotica sp. nov.发光杆菌属的多相分类及新分类单元的提议：发光光杆状菌发光亚种新亚种、发光光杆状菌阿克赫斯特亚种新亚种、发光光杆状菌拉蒙德亚种新亚种、温和光杆状菌新种、温和光杆状菌温和亚种新亚种及共生无光光杆状菌新种
Int J Syst Bacteriol. 1999 Oct;49 Pt 4:1645-56. doi: 10.1099/00207713-49-4-1645.
7
Influence of the ascarosides on the recovery, yield and dispersal of entomopathogenic nematodes.阿索昔康对昆虫病原线虫的恢复、产量和扩散的影响。
J Invertebr Pathol. 2022 Feb;188:107717. doi: 10.1016/j.jip.2022.107717. Epub 2022 Jan 11.
8
Photorhabdus luminescens subsp. kleinii subsp. nov. (Enterobacteriales: Enterobacteriaceae).发光杆菌亚属 kleinii 亚种（肠杆菌目：肠杆菌科）。
Curr Microbiol. 2011 Feb;62(2):539-43. doi: 10.1007/s00284-010-9741-z. Epub 2010 Aug 18.
9
Strains of Photorhabdus spp. associated with polish Heterorhabditis isolates: their molecular and phenotypic characterization and symbiont exchange.与波兰小卷蛾斯氏线虫分离株相关的发光杆菌菌株：其分子和表型特征及共生菌交换
Arch Microbiol. 2017 Sep;199(7):979-989. doi: 10.1007/s00203-017-1368-z. Epub 2017 Apr 5.
10
Molecular diversity of Photorhabdus and Xenorhabdus bacteria, symbionts of Heterorhabditis and Steinernema nematodes retrieved from soil in Benin.从贝宁土壤中分离得到的作为异小杆线虫和斯氏线虫共生菌的发光杆菌属和嗜线虫致病杆菌属细菌的分子多样性。
Arch Microbiol. 2018 May;200(4):589-601. doi: 10.1007/s00203-017-1470-2. Epub 2017 Dec 21.
引用本文的文献
1
Antifungal Substances Produced by and Its Inhibition Mechanism against .及其对 的抑制机制产生的抗真菌物质。
Int J Mol Sci. 2022 Aug 12;23(16):9040. doi: 10.3390/ijms23169040.
2
Screening of the Antimicrobial Activity against Drug Resistant Bacteria of and Associated with Entomopathogenic Nematodes from Mae Wong National Park, Thailand.泰国湄旺国家公园昆虫病原线虫及其相关细菌对耐药菌的抗菌活性筛选
Front Microbiol. 2017 Jun 28;8:1142. doi: 10.3389/fmicb.2017.01142. eCollection 2017.
3
Draft Genome Sequence and Annotation of the Entomopathogenic Bacterium Photorhabdus luminescens LN2, Which Shows Nematicidal Activity against Heterorhabditis bacteriophora H06 Nematodes.对发光光杆状菌LN2的基因组序列草案及注释，该菌株对嗜线虫致病杆菌H06线虫具有杀线虫活性。
Genome Announc. 2014 Dec 11;2(6):e01268-14. doi: 10.1128/genomeA.01268-14.
4
Comprehensive analysis of the HEPN superfamily: identification of novel roles in intra-genomic conflicts, defense, pathogenesis and RNA processing.全面分析 HEPN 超家族：鉴定其在基因组内冲突、防御、发病机制和 RNA 处理中的新作用。
Biol Direct. 2013 Jun 15;8:15. doi: 10.1186/1745-6150-8-15.
5
Genetic and proteomic characterization of rpoB mutations and their effect on nematicidal activity in Photorhabdus luminescens LN2.rpoB 基因突变的遗传和蛋白质组学特征及其对发光杆菌 LN2 杀线虫活性的影响。
PLoS One. 2012;7(8):e43114. doi: 10.1371/journal.pone.0043114. Epub 2012 Aug 17.
本文引用的文献
1
Mutualism and pathogenesis in Xenorhabdus and Photorhabdus: two roads to the same destination.嗜线虫致病杆菌属和发光杆菌属中的共生与致病机制：殊途同归。
Mol Microbiol. 2007 Apr;64(2):260-8. doi: 10.1111/j.1365-2958.2007.05671.x.
2
The effect of Photorhabdus luminescens (Enterobacteriaceae) on the survival, development, reproduction and behaviour of Caenorhabditis elegans (Nematoda: Rhabditidae).发光光杆状菌（肠杆菌科）对秀丽隐杆线虫（线虫纲：小杆线虫科）存活、发育、繁殖及行为的影响
Environ Microbiol. 2007 Jan;9(1):12-25. doi: 10.1111/j.1462-2920.2006.01099.x.
3
Photorhabdus virulence cassettes confer injectable insecticidal activity against the wax moth.嗜线虫致病杆菌毒力盒赋予对大蜡螟的可注射杀虫活性。
J Bacteriol. 2006 Mar;188(6):2254-61. doi: 10.1128/JB.188.6.2254-2261.2006.
4
Isolation of transposon mutants from Azospirillum brasilense Yu62 and characterization of genes involved in indole-3-acetic acid biosynthesis.从巴西固氮螺菌Yu62中分离转座子突变体并鉴定参与吲哚-3-乙酸生物合成的基因。
FEMS Microbiol Lett. 2005 Jul 1;248(1):57-63. doi: 10.1016/j.femsle.2005.05.020.
5
When mutualists are pathogens: an experimental study of the symbioses between Steinernema (entomopathogenic nematodes) and Xenorhabdus (bacteria).当互利共生者成为病原体时：斯氏线虫属（昆虫病原线虫）与嗜线虫致病杆菌属（细菌）共生关系的实验研究
J Evol Biol. 2004 Sep;17(5):985-93. doi: 10.1111/j.1420-9101.2004.00748.x.
6
The genome sequence of the entomopathogenic bacterium Photorhabdus luminescens.昆虫致病细菌发光杆菌的基因组序列。
Nat Biotechnol. 2003 Nov;21(11):1307-13. doi: 10.1038/nbt886. Epub 2003 Oct 5.
7
Use of a transposon with luciferase as a reporter to identify environmentally responsive genes in a cyanobacterium.使用带有荧光素酶作为报告基因的转座子来鉴定蓝细菌中的环境响应基因。
Proc Natl Acad Sci U S A. 1991 Jun 15;88(12):5355-9. doi: 10.1073/pnas.88.12.5355.
8
Effect of Photorhabdus luminescens phase variants on the in vivo and in vitro development and reproduction of the entomopathogenic nematodes Heterorhabditis bacteriophora and Steinernema carpocapsae.发光杆菌相变体对昆虫病原线虫嗜菌异小杆线虫和小卷蛾斯氏线虫体内外发育及繁殖的影响
FEMS Microbiol Ecol. 2001 May;35(3):239-247. doi: 10.1111/j.1574-6941.2001.tb00809.x.
9
Purification and characterization of a high-molecular-weight insecticidal protein complex produced by the entomopathogenic bacterium photorhabdus luminescens.昆虫病原细菌发光杆菌产生的一种高分子量杀虫蛋白复合物的纯化与特性分析
Appl Environ Microbiol. 1998 Aug;64(8):3029-35. doi: 10.1128/AEM.64.8.3029-3035.1998.
10
Insecticidal toxins from the bacterium Photorhabdus luminescens.来自发光杆菌的杀虫毒素。
Science. 1998 Jun 26;280(5372):2129-32. doi: 10.1126/science.280.5372.2129.
Zotero 插件