对参与发光杆菌LN2种间杀线虫活性的一个新基因的鉴定与表征
Identification and characterization of a novel gene involved in the trans-specific nematicidal activity of Photorhabdus luminescens LN2.
作者信息
Qiu Xuehong, Han Richou, Yan Xun, Liu Mingxing, Cao Li, Yoshiga Toyoshi, Kondo Eizo
机构信息
Sun Yat-sen University, Guangzhou, China.
出版信息
Appl Environ Microbiol. 2009 Jun;75(12):4221-3. doi: 10.1128/AEM.02967-08. Epub 2009 Apr 17.
Abstract
Photorhabdus luminescens subsp. akhurstii LN2 from Heterorhabditis indica LN2 showed nematicidal activity against axenic Heterorhabditis bacteriophora H06 infective juveniles (IJs). Transposon mutagenesis identified an LN2 mutant that supports the growth of H06 nematodes. Tn5 disrupted the namA gene, encoding a novel 364-residue protein and involving the nematicidal activity. The green fluorescent protein-labeled namA mutant was unable to colonize the intestines of H06 IJs.
摘要
来自印度异小杆线虫LN2的发光杆菌阿克赫斯特亚种LN2对无菌的嗜菌异小杆线虫H06感染性幼虫（IJs）表现出杀线虫活性。转座子诱变鉴定出一个支持H06线虫生长的LN2突变体。Tn5破坏了namA基因，该基因编码一种新的364个氨基酸残基的蛋白质，并与杀线虫活性有关。绿色荧光蛋白标记的namA突变体无法定殖在H06 IJs的肠道中。
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