Yi Huan, Zheng Xiangqin, Song Jianrong, Shen Rongkai, Su Yanzhao, Lin Danmei
Gynecological Oncology, Fujian Maternity and Children Health Hospital Fujian Medical University Teaching Hospital Fuzhou 350005, China.
The First Affiliated Hospital of Fujian Medical University Fuzhou 350005, China.
Int J Clin Exp Pathol. 2015 Dec 1;8(12):15719-28. eCollection 2015.
Epithelial ovarian cancer is the most lethal gynecological malignancies for readily metastasis. Exosomes have played an influential role in carcinogenicity and cancer progression. Our aim is to discover exosome-related mechanisms in ovarian cancer progress and explore potential diagnostic biomarkers and therapeutic targets of ovarian cancer. We initially presented the proteomic profiles of exosomes derived from two late-stage ovarian cell lines, OVCA429 and HO8910PM. A total of 2940 exosomal proteins were recorded by MS. FunRich appropriately processed these exosomal proteins, manifesting some superiority in contrast to Blast2go. Moreover, we demonstrated the pentose phosphate pathway was a dominant mechanism in exosome mediated intracellular communication. Glucose-6-phosphate dehydrogenase, transketolase and transaldolase 1, three key enzymes regulated pentose phosphate pathway, were all marked in the same exosomal parts of proteins between two ovarian cell lines. Moreover, these key proteins might become diagnostic, prognostic biomarkers and therapeutic targets of ovarian cancer.
上皮性卵巢癌是最致命的妇科恶性肿瘤，极易发生转移。外泌体在致癌性和癌症进展中发挥了重要作用。我们的目的是发现卵巢癌进展中与外泌体相关的机制，并探索卵巢癌潜在的诊断生物标志物和治疗靶点。我们最初展示了源自两种晚期卵巢癌细胞系OVCA429和HO8910PM的外泌体的蛋白质组学图谱。通过质谱记录了总共2940种外泌体蛋白。FunRich对这些外泌体蛋白进行了适当处理，与Blast2go相比显示出一些优势。此外，我们证明磷酸戊糖途径是外泌体介导的细胞内通讯的主要机制。葡萄糖-6-磷酸脱氢酶、转酮醇酶和转醛醇酶1这三种调节磷酸戊糖途径的关键酶，在两种卵巢癌细胞系的相同外泌体蛋白部分均有标记。此外，这些关键蛋白可能成为卵巢癌的诊断、预后生物标志物和治疗靶点。
