微小RNA-155表达作为胆囊癌患者手术切除后的预后因素
MicroRNA-155 expression as a prognostic factor in patients with gallbladder carcinoma after surgical resection.
作者信息
Zhang Xue-Lin, Chen Jun-Hong, Qin Cheng-Kun
机构信息
Department of General Surgery, Shandong Provincial Hospital, Shandong UniversityJinan 250021, Shandong, China; Department of General Surgery, Liaocheng People's HospitalLiaocheng 252000, Shandong, China.
Department of General Surgery, Liaocheng People's Hospital Liaocheng 252000, Shandong, China.
出版信息
Int J Clin Exp Med. 2015 Nov 15;8(11):21241-6. eCollection 2015.
BACKGROUND
MicroRNA-155 (miR-155) is over-expressed in both hematopoietic malignancies and solid tumors. In the present study, we investigated the clinical significance of miR-155 in gallbladder carcinoma among Chinese population.
METHODS
Tissue specimens were collected from 133 patients who had undergone surgical resection at Shandong Provincial Hospital, Shandong University between May 2008 and April 2014. We profiled miR- 155 expression in the gallbladder carcinoma tissues and normal gallbladder tissues by qRT-PCR. The Kaplan-Meier method was used to analyze the 5-year survival rate.
RESULTS
The expression levels of miR-155 were significantly higher in gallbladder carcinoma tissues than that in normal gallbladder tissues (P<0.001). High miR-155 expression was significantly associated with TNM stage (P=0.003), lymph node status (P=0.042), liver metastasis (P=0.010), and differentiated degree (P<0.001). We found that gallbladder carcinoma patients with high miR-155 expression level had distinctly shorter overall survival than patients with low miR-155 expression level (P=0.03). Multivariate analysis revealed that miR-155 expression level was independent prognostic factors for overall survival (HR=2.394, 95% CI: 1.568-10.034; P=0.009).
CONCLUSION
High miR-155 expression is a prognostic indicator for poor prognosis of patients with gallbladder carcinoma among Chinese population.
背景
微小RNA-155（miR-155）在血液系统恶性肿瘤和实体瘤中均呈过表达。在本研究中，我们调查了miR-155在中国人群胆囊癌中的临床意义。
方法
收集了2008年5月至2014年4月在山东大学齐鲁医院接受手术切除的133例患者的组织标本。我们通过qRT-PCR检测了胆囊癌组织和正常胆囊组织中miR-155的表达情况。采用Kaplan-Meier法分析5年生存率。
结果
miR-155在胆囊癌组织中的表达水平显著高于正常胆囊组织（P<0.001）。miR-155高表达与TNM分期（P=0.003）、淋巴结状态（P=0.042）、肝转移（P=0.010）及分化程度（P<0.001）显著相关。我们发现，miR-155表达水平高的胆囊癌患者的总生存期明显短于miR-155表达水平低的患者（P=0.03）。多因素分析显示，miR-155表达水平是总生存期的独立预后因素（HR=2.394，95%CI：1.568-10.034；P=0.009）。
结论
miR-155高表达是中国人群胆囊癌患者预后不良的一个预后指标。
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