Lv Zhonghua, Zhao Yashuang
Department of Neurosurgery, The Third Affiliated Hospital of Harbin Medical University, Heilongjiang Institute for Cancer Research Harbin, Heilongjiang, P. R. China.
School of Public Health, Harbin Medical University Harbin, Heilongjiang, P. R. China.
Int J Clin Exp Pathol. 2017 Nov 1;10(11):11369-11376. eCollection 2017.
Tumorigenesis research has focused on the roles of deregulated microRNAs for many years. The aberrant expression of miR-124 in many tumors has been widely reported, yet its role in glioma formation still needs further research. In this study, the expression and mechanisms of miR-124 in glioma development were explored. We found that glioma cell lines and tumor tissues demonstrated downregulated miR-124 expression, and that cell proliferation, migration, and invasion were reduced when miR-124 was restored. Furthermore, a bioinformatic analysis indicated that Smad2 was a putative target of miR-124, and we confirmed that miR-124 directly targets Smad2 in a luciferase reporter assay system. These results indicate that glioma cell growth is suppressed by miR-124 through its negative regulation of Smad2 expression. Our findings disclose a critical role of miR-124 in glioma pathogenesis, and suggest its potential application for glioma therapy.
多年来，肿瘤发生研究一直聚焦于失调的微小RNA的作用。许多肿瘤中miR-124的异常表达已被广泛报道，但其在胶质瘤形成中的作用仍需进一步研究。在本研究中，我们探讨了miR-124在胶质瘤发展中的表达及机制。我们发现，胶质瘤细胞系和肿瘤组织中miR-124表达下调，恢复miR-124表达后，细胞增殖、迁移和侵袭能力降低。此外，生物信息学分析表明，Smad2是miR-124的一个潜在靶点，我们在荧光素酶报告基因检测系统中证实了miR-124直接靶向Smad2。这些结果表明，miR-124通过对Smad2表达的负调控抑制胶质瘤细胞生长。我们的研究结果揭示了miR-124在胶质瘤发病机制中的关键作用，并提示其在胶质瘤治疗中的潜在应用价值。
