School of Environmental Sciences, Jawaharlal Nehru University, New Delhi 110067, India.
School of Environmental Sciences, Jawaharlal Nehru University, New Delhi 110067, India.
Ecotoxicol Environ Saf. 2016 Jun;128:44-51. doi: 10.1016/j.ecoenv.2016.02.009. Epub 2016 Feb 15.
The present study aims to evaluate the feasibility of leachate treatment using a synergistic approach by microalgae and bacteria. Leachate from one of the landfill of Northern India showed the presence of various toxic organic contaminants like naphthalene, benzene, phenol and their derivatives, napthols, pesticides, epoxides, phthalates and halogenated organic compounds. ICP-AES analysis revealed high concentrations of Zn, Cr, Fe, Ni, and Pb beyond the maximum permissible limit of discharge. Bacto-algal co-culture was found to be the most efficient in removal of toxic organic contaminants and heavy metals. Further, detoxification efficiency of bacto-algal treatment was evaluated by Methyl tetrazolium (MTT) assay for cytotoxicity and alkaline comet assay for genotoxicity using hepatoma HepG2 cells. Reduction in toxicity was confirmed by an increase in LC50 by 1.9 fold and reduction in Olive Tail Moment by 40.6 fold after 10 days of treatment. Results of the study indicate bioremediation and detoxification potency of bacto-algal co-culture for leachate treatment.
本研究旨在评估微藻和细菌协同作用处理渗滤液的可行性。来自印度北部一个垃圾填埋场的渗滤液中存在各种有毒有机污染物，如萘、苯、苯酚及其衍生物、萘酚、农药、环氧化物、邻苯二甲酸酯和卤代有机化合物。ICP-AES 分析显示，Zn、Cr、Fe、Ni 和 Pb 的浓度超过了最大允许排放限值。研究发现，细菌-藻类共培养在去除有毒有机污染物和重金属方面最为有效。此外，还通过 MTT 法测定细胞毒性和碱性彗星试验测定遗传毒性，评估了细菌-藻类处理的解毒效率，分别使用肝癌 HepG2 细胞。通过 10 天的处理，LC50 增加了 1.9 倍，Olive Tail Moment 减少了 40.6 倍，证实了毒性的降低。研究结果表明，细菌-藻类共培养具有渗滤液处理的生物修复和解毒能力。
