Brustolin M, Talavera S, Santamaría C, Rivas R, Pujol N, Aranda C, Marquès E, Valle M, Verdún M, Pagès N, Busquets N
Centre de Recerca en Sanitat Animal (CReSA), Institut de Recerca i Tecnologia Agroalimentàries (IRTA), Universitat Autònoma de Barcelona, Barcelona, Spain.
Servei de Control de Mosquits, Consell Comarcal del Baix Llobregat, Barcelona, Spain.
Med Vet Entomol. 2016 Jun;30(2):166-73. doi: 10.1111/mve.12164. Epub 2016 Feb 18.
The emerging disease West Nile fever is caused by West Nile virus (WNV), one of the most widespread arboviruses. This study represents the first test of the vectorial competence of European Culex pipiens Linnaeus 1758 and Stegomyia albopicta (= Aedes albopictus) (both: Diptera: Culicidae) populations for lineage 1 and 2 WNV isolated in Europe. Culex pipiens and S. albopicta populations were susceptible to WNV infection, had disseminated infection, and were capable of transmitting both WNV lineages. This is the first WNV competence assay to maintain mosquito specimens under environmental conditions mimicking the field (day/night) conditions associated with the period of maximum expected WNV activity. The importance of environmental conditions is discussed and the issue of how previous experiments conducted in fixed high temperatures may have overestimated WNV vector competence results with respect to natural environmental conditions is analysed. The information presented should be useful to policymakers and public health authorities for establishing effective WNV surveillance and vector control programmes. This would improve preparedness to prevent future outbreaks.
新兴疾病西尼罗河热由西尼罗河病毒（WNV）引起，该病毒是传播最广泛的虫媒病毒之一。本研究首次检测了欧洲1758年林奈库蚊和白纹伊蚊（均属双翅目：蚊科）种群对在欧洲分离出的1型和2型WNV的媒介能力。库蚊和白纹伊蚊种群易感染WNV，有传播感染，并且能够传播两种WNV谱系。这是首次在模拟与预期WNV活动高峰期相关的野外（白天/夜晚）环境条件下饲养蚊虫标本进行的WNV能力测定。文中讨论了环境条件的重要性，并分析了以往在固定高温下进行的实验可能如何在自然环境条件方面高估了WNV媒介能力结果这一问题。所提供的信息对于政策制定者和公共卫生当局制定有效的WNV监测和病媒控制计划应是有用的。这将提高预防未来疫情爆发的准备能力。
