Nakatsuji N, Nagata I
Division of Developmental Biology, Meiji Institute of Health Science, Odawara, Japan.
Development. 1989 Jul;106(3):441-7. doi: 10.1242/dev.106.3.441.
In order to study migration of neurons in vitro, we cultured microexplants of the newborn mouse cerebellum outer layer, which is rich in immature granule cells, on a substratum double-coated with poly-L-lysine and laminin. The granule cells first migrated away from the explant along radially oriented parallel bundles of their neurites, thus displaying typical contact guidance. Then, in almost all explants, they changed their orientation by 90 degrees to extend cell processes and translocate perpendicular to the radial neurites. Orientation and migration of neurons perpendicular to the aligned parallel structure is a novel type of contact-guided cell behavior, and may have interesting implications in migration of neurons in the cerebellum and other parts of the nervous system.
为了在体外研究神经元的迁移，我们将富含未成熟颗粒细胞的新生小鼠小脑外层的微组织块培养在涂有聚-L-赖氨酸和层粘连蛋白的双层基质上。颗粒细胞首先沿着其神经突的径向平行束从组织块迁移开，从而表现出典型的接触导向。然后，在几乎所有的组织块中，它们将方向改变90度以延伸细胞突起并垂直于径向神经突进行移位。神经元垂直于排列的平行结构的定向和迁移是一种新型的接触导向细胞行为，并且可能对小脑和神经系统其他部位的神经元迁移具有有趣的影响。
