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用于表征微生物表面的物理方法。

Physical methods for characterization of microbial surfaces.

作者信息

Krekeler C, Ziehr H, Klein J

机构信息

GBF, Gesellschaft für Biotechnologische Forschung, Braunschweig, Federal Republic of Germany.

出版信息

Experientia. 1989 Dec 1;45(11-12):1047-55. doi: 10.1007/BF01950157.

DOI:10.1007/BF01950157
PMID:2689202
Abstract

There are different concepts for explaining the adsorption of microorganisms to solid surfaces: the DLVO theory and the surface free energy. Basic aspects of both theories are discussed. Established methods for determining the surface properties of microbial cells are reviewed: Electrophoretic mobility, colloid titration, electrostatic interaction chromatography, bacterial adherence to hydrocarbons, partitioning in an aqueous two-phase system, hydrophobic interaction chromatography, contact angle measurement and X-ray photoelectron spectroscopy. They are discussed and classified according to their potential for the correlation of cell surface characteristics and adsorption behavior.

摘要

对于解释微生物在固体表面的吸附现象，存在不同的概念：DLVO理论和表面自由能。本文讨论了这两种理论的基本方面。综述了用于测定微生物细胞表面性质的既定方法：电泳迁移率、胶体滴定、静电相互作用色谱法、细菌对烃类的粘附、在水两相系统中的分配、疏水相互作用色谱法、接触角测量和X射线光电子能谱。根据它们在关联细胞表面特征和吸附行为方面的潜力进行了讨论和分类。

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Immobilization of microorganisms by adhesion: interplay of electrostatic and nonelectrostatic interactions.通过粘附固定微生物：静电和非静电相互作用的相互影响
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Changes in the Electrokinetic Potential of Bacteria at Various Phases of the Culture Cycle.细菌在培养周期不同阶段的动电电位变化
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Starvation-induced effects on bacterial surface characteristics.饥饿对细菌表面特性的影响。
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Protein Adsorption Patterns and Analysis on IV Nanoemulsions-The Key Factor Determining the Organ Distribution.蛋白吸附模式及 IV 纳米乳分析-决定器官分布的关键因素。
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Electrophoresis of Micrococcus pyogenes aureus.化脓性金黄色微球菌的电泳
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The isoelectric point of bacterial cells.细菌细胞的等电点。
J Bacteriol. 1953 Feb;65(2):198-202. doi: 10.1128/jb.65.2.198-202.1953.