Tang X J, Hepler P K, Scordilis S P
Department of Biochemistry, Beijing Agricultural University, China.
J Cell Sci. 1989 Apr;92 ( Pt 4):569-74. doi: 10.1242/jcs.92.4.569.
A myosin heavy chain polypeptide has been identified and localized in Nicotiana pollen tubes using monoclonal anti-myosin antibodies. The epitopes of these antibodies were found to reside on the myosin heavy chain head and rod portion and were, therefore, designated anti-S-1 (myosin S-1) and anti-LMM (light meromyosin). On Western blots of the total soluble pollen tube proteins, both anti-S-1 and anti-LMM label a polypeptide of approximately 175,000 Mr. Immunofluorescence microscopy shows that both antibodies yield numerous fluorescent spots throughout the whole length of the tube, often with an enrichment in the tube tip. These fluorescent spots are thought to represent vesicles and/or organelles in the pollen tubes. In addition to this common pattern, anti-S-1 stains both the generative cell and the vegetative nuclear envelope. The different staining patterns of the nucleus between anti-S-1 and anti-LMM may be caused by some organization and/or anchorage state of the myosin molecules on the nuclear surface that differs from those on the vesicles and/or organelles.
利用单克隆抗肌球蛋白抗体，在烟草花粉管中鉴定并定位了一种肌球蛋白重链多肽。发现这些抗体的表位位于肌球蛋白重链头部和杆状部分，因此被命名为抗S-1（肌球蛋白S-1）和抗LMM（轻酶解肌球蛋白）。在花粉管总可溶性蛋白的蛋白质免疫印迹上，抗S-1和抗LMM均标记出一条分子量约为175,000的多肽。免疫荧光显微镜检查显示，两种抗体在整个花粉管长度上产生大量荧光斑点，且在管尖处常常更为密集。这些荧光斑点被认为代表花粉管中的囊泡和/或细胞器。除了这种共同模式外，抗S-1还对生殖细胞和营养细胞核膜进行染色。抗S-1和抗LMM对细胞核的不同染色模式可能是由核表面肌球蛋白分子的某些组织和/或锚定状态与囊泡和/或细胞器上的不同所致。
