Wang Weicang, Du Zheyuan, Nimiya Yoshiki, Sukamtoh Elvira, Kim Daeyoung, Zhang Guodong
Department of Food Science, University of Massachusetts, Amherst, MA, USA.
Department of Food Science, University of Massachusetts, Amherst, MA, USA; Department of Food Science and Technology, Tokyo University of Marine Science and Technology, Tokyo, Japan.
J Nutr Biochem. 2016 Mar;29:83-9. doi: 10.1016/j.jnutbio.2015.11.004. Epub 2015 Nov 24.
Allicin, the most abundant organosulfur compound in freshly crushed garlic tissues, has been shown to have various health-promoting effects, including anticancer actions. A better understanding of the effects and mechanisms of allicin on tumorigenesis could facilitate development of allicin or garlic products for cancer prevention. Here we found that allicin inhibited lymphangiogenesis, which is a critical cellular process implicated in tumor metastasis. In primary human lymphatic endothelial cells, allicin at 10 μM inhibited capillary-like tube formation and cell migration, and it suppressed phosphorylation of vascular endothelial growth factor receptor 2 and focal adhesion kinase. Using a Matrigel plug assay in mice, addition of 10 μg allicin in Matrigel plug inhibited 40-50% of vascular endothelial growth factor-C-induced infiltration of lymphatic endothelial cells and leukocytes. S-Allylmercaptoglutathione, a major cellular metabolite of allicin, had no effect on lymphangiogenic responses in lymphatic endothelial cells. Together, these results demonstrate the antilymphangiogenic effect of allicin in vitro and in vivo, suggesting a novel mechanism for the health-promoting effects of garlic compounds.
大蒜素是新鲜碾碎的大蒜组织中含量最丰富的有机硫化合物，已被证明具有多种促进健康的作用，包括抗癌作用。更好地了解大蒜素对肿瘤发生的影响和机制，有助于开发用于癌症预防的大蒜素或大蒜产品。在此，我们发现大蒜素可抑制淋巴管生成，而淋巴管生成是肿瘤转移所涉及的关键细胞过程。在原代人淋巴管内皮细胞中，10 μM的大蒜素可抑制毛细血管样管形成和细胞迁移，并抑制血管内皮生长因子受体2和粘着斑激酶的磷酸化。在小鼠中使用基质胶栓试验，在基质胶栓中添加10 μg大蒜素可抑制血管内皮生长因子C诱导的淋巴管内皮细胞和白细胞浸润的40%-50%。大蒜素的主要细胞代谢产物S-烯丙基巯基谷胱甘肽对淋巴管内皮细胞的淋巴管生成反应没有影响。总之，这些结果证明了大蒜素在体外和体内的抗淋巴管生成作用，提示了大蒜化合物促进健康作用的一种新机制。
