Key Laboratory of Industrial Ecology and Environmental Engineering, Ministry of Education, School of Environmental Science and Technology, Dalian University of Technology, Linggong Road 2, Dalian, Liaoning 116024, China.
Key Laboratory of Industrial Ecology and Environmental Engineering, Ministry of Education, School of Environmental Science and Technology, Dalian University of Technology, Linggong Road 2, Dalian, Liaoning 116024, China.
Bioresour Technol. 2016 May;207:308-14. doi: 10.1016/j.biortech.2016.02.005. Epub 2016 Feb 6.
An up-flow packed-bed bioreactor was constructed to investigate the simultaneous removal of chromate and nitrate using biodegradable meal box as carbon source and biofilm carriers. The bioreactor was operated for 164days with varying influent Cr(VI) concentrations (2.0-50.0mg/L) and hydraulic retention times (HRT, 10-24h). It was shown that complete denitrification and Cr(VI) reduction could be achieved when influent Cr(VI) concentrations were lower than 20mg/L with a HRT of 17h. Shortening the HRT could significantly reduce the effluent CODcr. It was also observed that Cr(III) was mainly immobilized on the biofilm. Further investigation on Cr distribution in the biofilm compartments indicated that Cr(VI) reduction occurred in all compartments and the intercellular Cr was dominant. High-throughput sequencing analysis showed that Proteobacteria, Bacteroidetes and Firmicutes were the dominant phyla in the biofilm and Cr(VI) stress had a negative effect on the abundance of most bacteria.
上流式填充床生物反应器被构建用于研究使用可生物降解餐盒作为碳源和生物膜载体同时去除铬酸盐和硝酸盐。该生物反应器运行了 164 天，进水 Cr(VI)浓度（2.0-50.0mg/L）和水力停留时间（HRT，10-24h）不同。结果表明，当进水 Cr(VI)浓度低于 20mg/L 且 HRT 为 17h 时，可以实现完全反硝化和 Cr(VI)还原。缩短 HRT 可以显著降低出水 CODcr。还观察到 Cr(III)主要被固定在生物膜上。对生物膜各隔室中 Cr 分布的进一步研究表明，Cr(VI)还原发生在所有隔室中，细胞间 Cr 占主导地位。高通量测序分析表明，在生物膜中优势菌门为变形菌门、拟杆菌门和厚壁菌门，Cr(VI)胁迫对大多数细菌的丰度有负面影响。
