Martins Christine Men, Gomes-Filho João Eduardo, de Azevedo Queiroz Índia Olinta, Ervolino Edilson, Cintra Luciano Tavares Angelo
Restorative Dentistry Department, Endodontic Area, Aracatuba Dental School, Universidade Estadual Paulista, Aracatuba, São Paulo, Brazil.
Restorative Dentistry Department, Endodontic Area, Aracatuba Dental School, Universidade Estadual Paulista, Aracatuba, São Paulo, Brazil.
J Endod. 2016 Apr;42(4):604-9. doi: 10.1016/j.joen.2016.01.003. Epub 2016 Feb 18.
This study evaluated the effect of hypertension on tissue response to and mineralization capacity of white and gray mineral trioxide aggregate (MTA) (Angelus Industry Ontological Products, Londrina, Brazil), an endodontic reparative cement.
Polyethylene tubes containing gray MTA, white MTA, or intermediate restorative material (positive control) or an empty tube (negative control) were implanted into the dorsal connective tissue of spontaneous hypertensive and Wistar rats (n = 12 each). Six rats in each group were sacrificed after 7 days, and the remainder after 30 days. Tubes with surrounding tissue were removed, and a histologic analysis was performed using hematoxylin-eosin and von Kossa staining and examination by polarized light microscopy.
The inflammatory response to all materials was greater in hypertensive compared with normotensive rats (P < .05). Positive von Kossa staining and birefringent structures in polarized light were observed for both gray and white MTA (P > .05), but these were more pronounced in normotensive rats (P < .05). Necrotic areas with positive von Kossa staining were observed for intermediate restorative material.
Hypertension undermines tissue repair and mineralization, which can negatively affect treatment outcome. Nonetheless, mineralization in response to MTA was observed even under hypertensive conditions.
本研究评估了高血压对白色和灰色三氧化矿物凝聚体（MTA）（巴西隆德里纳的安热卢斯工业本体产品公司生产）这一牙髓修复用黏固剂的组织反应及矿化能力的影响。
将装有灰色MTA、白色MTA、中间修复材料（阳性对照）或空管（阴性对照）的聚乙烯管植入自发性高血压大鼠和Wistar大鼠（每组各12只）的背部结缔组织中。每组6只大鼠在7天后处死，其余在30天后处死。取出带有周围组织的管子，使用苏木精-伊红染色和冯·科萨染色进行组织学分析，并通过偏光显微镜检查。
与正常血压大鼠相比，高血压大鼠对所有材料的炎症反应更大（P < 0.05）。灰色和白色MTA均观察到阳性冯·科萨染色和偏光下的双折射结构（P > 0.05），但在正常血压大鼠中更明显（P < 0.05）。中间修复材料观察到有阳性冯·科萨染色的坏死区域。
高血压会破坏组织修复和矿化，这可能对治疗结果产生负面影响。尽管如此，即使在高血压条件下，对MTA的矿化反应依然可见。
