Arnold Philipp, Rickert Uta, Helmers Ann-Kristin, Spreu Jessica, Schneppenheim Janna, Lucius Ralph
Anatomical Institute, Otto-Hahn Platz 8, 24188, Kiel, Germany.
Institute of Neurosurgery UKSH Kiel, Arnold-Heller-Straße 3, 24105, Kiel, Germany.
Cell Tissue Res. 2016 Jul;365(1):3-11. doi: 10.1007/s00441-016-2370-5. Epub 2016 Feb 22.
Microglial cells are a major source of pro-inflammatory cytokines during central nervous system (CNS) inflammation. They can develop a pro-inflammatory M1 phenotype and an anti-inflammatory M2 phenotype. Shifting the phenotype from M1 to M2 might be an important mechanism to overcome CNS inflammation and to prevent or reduce neuronal damage. Here, we demonstrate that the anti-inflammatory protein trefoil factor 3 (TFF3) is secreted by astrocytes and that its transcription is significantly reduced after incubation with lipopolysaccharide (LPS). Moreover, we demonstrate that microglial cells cultured in the presence of TFF3 show reduced expression and secretion of pro-inflammatory cytokines after LPS stimulation.
小胶质细胞是中枢神经系统（CNS）炎症期间促炎细胞因子的主要来源。它们可以发展出促炎的M1表型和抗炎的M2表型。将表型从M1转变为M2可能是克服CNS炎症以及预防或减少神经元损伤的重要机制。在此，我们证明抗炎蛋白三叶因子3（TFF3）由星形胶质细胞分泌，并且在用脂多糖（LPS）孵育后其转录显著降低。此外，我们证明在TFF3存在下培养的小胶质细胞在LPS刺激后促炎细胞因子的表达和分泌减少。
