Chao Owen Y, Huston Joseph P, Nikolaus Susanne, de Souza Silva Maria A
Center for Behavioral Neuroscience, University of Düsseldorf, Universitätsstr. 1, 40225 Düsseldorf, Germany.
Clinic of Nuclear Medicine, University Hospital Düsseldorf, 40225 Düsseldorf, Germany.
Neurobiol Learn Mem. 2016 Apr;130:149-58. doi: 10.1016/j.nlm.2016.02.007. Epub 2016 Feb 17.
We here explore the utility of a paradigm that allows the simultaneous assessment of memory for object (what) and object location (where) and their comparative predominance. Two identical objects are presented during a familiarity trial; during the test trial one of these is displaced, and a new object is presented in a familiar location. When tested 5 or 80min later, rats explored both the novel and the displaced objects more than two familiar stationary objects, indicating intact memory for both, object and place. When tested 24h later rats explored the novel object more than the displaced familiar one, suggesting that forgetting differently influenced object and place memory, with memory for object being more robust than memory for place. Animals that received post-trial administration of the neurokinin-3 receptor agonist senktide and were tested 24h later, now explored the novel and displaced objects equally, suggesting that the treatment prevented the selective decay of memory for location. Next, animals received NMDA lesions in either the perirhinal cortex or the hippocampus, which are hypothesized to be preferentially involved in memory for objects and memory for place, respectively. When tested 5 or 80min later, the perirhinal cortex lesion group explored the displaced object more, indicating relatively deficient object memory, while the hippocampal lesion led to the opposite pattern, demonstrating comparatively deficient place memory. These results suggest different preferential engagement of the perirhinal cortex and hippocampus in their processing of memory for object and place. This preference test lends itself to application in the comparison of selective lesions of neural sites and projection systems as well as to the assessment of possible preferential action of pharmacological agents on neurochemical processes that subserve object vs place learning.
我们在此探讨一种范式的效用，该范式能够同时评估对物体（是什么）和物体位置（在哪里）的记忆及其相对优势。在熟悉性试验中呈现两个相同的物体；在测试试验中，其中一个物体被移动，并且在一个熟悉的位置呈现一个新物体。在5分钟或80分钟后进行测试时，大鼠对新奇物体和被移动的物体的探索多于两个熟悉的静止物体，表明对物体和位置都有完整的记忆。在24小时后进行测试时，大鼠对新奇物体的探索多于被移动的熟悉物体，这表明遗忘对物体和位置记忆的影响不同，物体记忆比位置记忆更持久。在试验后接受神经激肽-3受体激动剂senktide给药并在24小时后进行测试的动物，现在对新奇物体和被移动物体的探索相同，这表明该处理阻止了位置记忆的选择性衰退。接下来，动物在鼻周皮质或海马体接受NMDA损伤，据推测这两个区域分别优先参与物体记忆和位置记忆。在5分钟或80分钟后进行测试时，鼻周皮质损伤组对被移动物体的探索更多，表明物体记忆相对不足，而海马体损伤导致相反的模式，表明位置记忆相对不足。这些结果表明鼻周皮质和海马体在处理物体和位置记忆时具有不同的优先参与情况。这种偏好测试适用于比较神经位点和投射系统的选择性损伤，以及评估药物制剂对支持物体与位置学习的神经化学过程可能的优先作用。
