Fang Huimin, Meng Qingling, Zhang Hongsheng, Huang Ji
a State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University , Nanjing , China.
Bioengineered. 2016;7(1):39-45. doi: 10.1080/21655979.2015.1131368.
The plant-specific RING-domain finger proteins play important roles in plant development and stress responses. We recently identified and functionally characterized a stress-induced gene OsSRFP1 (Oryza sativa Stress-related RING Finger Protein 1) from rice. We showed evidences of the biotechnological potential of the suppression of OsSRFP1 expression in conferring cold tolerance. The increased cold tolerance of OsSRFP1 knock-down plants was associated with higher amounts of free proline and activities of antioxidant enzymes. In vitro ubiquitination assays showed that OsSRFP1 possessed E3 ubiquitin ligase activity. Some predicted interacting partners of OsSRFP1 might be the substrates for OsSRFP1-mediated protein degradation. Interestingly, OsSRFP1 had trans-activation activity, suggesting the dual roles of OsSRFP1 in post-translational and transcriptional regulations in stress responses.
植物特有的RING结构域蛋白在植物发育和胁迫响应中发挥着重要作用。我们最近从水稻中鉴定并对一个胁迫诱导基因OsSRFP1（水稻胁迫相关RING指蛋白1）进行了功能表征。我们展示了抑制OsSRFP1表达在赋予耐寒性方面的生物技术潜力的证据。OsSRFP1基因敲除植物耐寒性的提高与游离脯氨酸含量的增加和抗氧化酶的活性有关。体外泛素化分析表明OsSRFP1具有E3泛素连接酶活性。一些预测的OsSRFP1相互作用伙伴可能是OsSRFP1介导的蛋白质降解的底物。有趣的是，OsSRFP1具有反式激活活性，这表明OsSRFP1在胁迫响应的翻译后和转录调控中具有双重作用。
