Li An-Na, Li Sha, Li Ya, Xu Dong-Ping, Li Hua-Bin
Guangdong Provincial Key Laboratory of Food, Nutrition and Health, School of Public Health, Sun Yat-Sen University, Guangzhou 510080, China.
School of Chinese Medicine, The University of Hong Kong, Hong Kong, China.
Molecules. 2016 Feb 18;21(2):218. doi: 10.3390/molecules21020218.
An ultrasound-assisted extraction (UAE) method was developed to extract natural antioxidants from the Osmanthus fragrans flower. The effect of UAE on antioxidant activity of the extract from the Osmanthus fragrans flower was studied using a Trolox equivalent antioxidant capacity (TEAC) assay. Optimization conditions were firstly determined using a single-factor experiment, and response surface methodology was then used to evaluate interaction of several experimental parameters. Analysis of the coefficient of determination showed that second-order polynomial models produced a highly satisfactory fitting of the experimental data with regard to TEAC values (R² = 0.9829, p < 0.0001). The optimal conditions were 39.1% ethanol, and extraction for 35.2 min at 59.4 °C. Under these conditions, the maximum TEAC value was 584.9 ± 6.0 μmol Trolox/g DW, which was higher than those obtained by the conventional extracting method (486.4 ± 12.6 μmol Trolox/g DW) and the Soxhlet extraction method (339.1 ± 16.2 μmol Trolox/g DW). The crude extract obtained could be used either as a food additive or in pharmaceuticals for the prevention and treatment of diseases caused by oxidative stress.
开发了一种超声辅助提取（UAE）方法，用于从桂花中提取天然抗氧化剂。采用Trolox等效抗氧化能力（TEAC）测定法研究了UAE对桂花提取物抗氧化活性的影响。首先通过单因素实验确定优化条件，然后使用响应面法评估几个实验参数之间的相互作用。决定系数分析表明，二阶多项式模型对TEAC值的实验数据拟合度非常令人满意（R² = 0.9829，p < 0.0001）。最佳条件为39.1%乙醇，在59.4℃下提取35.2分钟。在此条件下，最大TEAC值为584.9±6.0 μmol Trolox/g DW，高于传统提取方法（486.4±12.6 μmol Trolox/g DW）和索氏提取法（339.1±16.2 μmol Trolox/g DW）获得的值。所得粗提物可作为食品添加剂或用于预防和治疗由氧化应激引起的疾病的药物中。
