Guangdong Provincial Key Laboratory of Food, Nutrition and Health, School of Public Health, Sun Yat-Sen University, Guangzhou 510080, China.
Ultrason Sonochem. 2012 Jul;19(4):772-6. doi: 10.1016/j.ultsonch.2011.11.014. Epub 2011 Dec 2.
Oleanolic acid and ursolic acid are the main bioactive compounds in fruit of Ligustrum lucidum Ait, which possess anti-inflammatory, antioxidative, antiprotozoal, antimutagenic and anticancer properties. In this study, the ultrasound-assisted extraction of oleanolic acid and ursolic acid from L. lucidum Ait was investigated with HPLC-photodiode array detection. Effects of several experimental parameters, such as type and concentration of extraction solvent, ratio of liquid to material, extraction temperature and extraction time, on extraction efficiencies of oleanolic acid and ursolic acid from L. lucidum were evaluated. The influence of experimental parameters on extraction efficiency of ursolic acid was more significant than that of oleanolic acid. The optimal extraction conditions were 95% ethanol, the ratio of material to liquid at 1:20, and extraction for 10 min at 40°C under ultrasonic irradiation. Under the optimal conditions, the yields of oleanolic acid and ursolic acid were 6.3 ± 0.25 and 9.8 ± 0.30 mg/g, respectively. The results indicated that the ultrasound-assisted extraction is a very useful method for the extraction of oleanolic acid and ursolic acid from L. lucidum.
齐墩果酸和熊果酸是女贞子果实中的主要生物活性化合物，具有抗炎、抗氧化、抗原生动物、抗突变和抗癌特性。本研究采用高效液相色谱-光电二极管阵列检测法研究了超声辅助提取女贞子中齐墩果酸和熊果酸的方法。考察了提取溶剂的种类和浓度、液料比、提取温度和提取时间等几个实验参数对女贞子中齐墩果酸和熊果酸提取效率的影响。实验参数对熊果酸提取效率的影响比对齐墩果酸的影响更为显著。最佳提取条件为 95%乙醇，料液比为 1:20，超声辐射下于 40°C 提取 10min。在最佳条件下，齐墩果酸和熊果酸的产率分别为 6.3±0.25mg/g 和 9.8±0.30mg/g。结果表明，超声辅助提取是一种从女贞子中提取齐墩果酸和熊果酸的非常有用的方法。
