Abdelhalim Mak
Department of Physics and Astronomy, College of Science, King Saud University, Saudi Arabia. E-mail:
West Indian Med J. 2015 May 7;65(1):87-92. doi: 10.7727/wimj.2014.350.
Gold nanoparticle (GNP) levels in the blood of rats have not been previously documented. This study aimed to evaluate the influence of size and exposure duration of GNPs on the gold levels in the blood of rats .
Thirty rats were divided into five groups, NG = control group, G1A: infusion of 10 nm GNPs for three days, G1B: 10 nm GNPs for seven days, G2A: 50 nm GNPs for three days and G2B: 50 nm GNPs for seven days. Fifty microlitres of GNPs dissolved in aqueous solution were administered intraperitoneally every day for three and seven days. Gold concentrations in different samples were measured using inductively coupled plasma-optical emission spectrometer (ICP-OES).
The percentage normalized value of 50nm GNPs increased in the blood of rats in both three and seven days when compared with 10 nm GNPs.
It became evident from the results of this study that the alterations in GNP levels were size dependent, with the smaller ones inducing the most toxic effects and related to the time of exposure of GNPs. The results might indicate that the smaller GNPs are mostly taken up and accumulate in the different rat organs, suggesting their toxic effects, while the 50 nm GNPs are retained in the blood of rats for a long time. These conclusions are further supported by the histological investigation.
此前尚未记录大鼠血液中的金纳米颗粒（GNP）水平。本研究旨在评估GNP的尺寸和暴露持续时间对大鼠血液中金含量的影响。
将30只大鼠分为五组，NG = 对照组，G1A：输注10纳米的GNP，持续三天，G1B：10纳米的GNP，持续七天，G2A：50纳米的GNP，持续三天，G2B：50纳米的GNP，持续七天。每天腹腔注射50微升溶解在水溶液中的GNP，持续三天和七天。使用电感耦合等离子体发射光谱仪（ICP-OES）测量不同样品中的金浓度。
与10纳米的GNP相比，50纳米的GNP在大鼠血液中的归一化值百分比在三天和七天时均有所增加。
从本研究结果可以明显看出，GNP水平的变化取决于尺寸，较小的GNP诱导的毒性作用最大，且与GNP的暴露时间有关。结果可能表明，较小的GNP大多被不同的大鼠器官摄取和积累，表明它们具有毒性作用，而50纳米的GNP则长时间保留在大鼠血液中。组织学研究进一步支持了这些结论。
