Prichard R K
Parasitology. 1978 Jun;76(3):277-88. doi: 10.1017/s0031182000048150.
Sheep infected with adult Fasciola hepatica were drenched with rafoxanide. At 4, 8, 16 and 24 h after drenching the sheep were killed and the flukes removed, washed and rapidly frozen in liquid nitrogen. The content of key metabolites in the fermentation pathway were determined and compared with those in control F. hepatica, whose hosts were not treated with rafoxanide. Rafoxanide decreased glycogen, malate, NADH and ATP levels. The level of other metabolites in the pathway increased for the first 8-16 h after rafoxanide treatment. The marked decrease in ATP and glycogen, and the increase in total [NAD+]/[NADH] and [oxaloacetate]/[malate], together with the changed content of other metabolites, led to the conclusion that the mode of action of rafoxanide against F. hepatica in vivo is by uncoupling oxidative phosphorylation.
用雷复尼特给感染了肝片形吸虫成虫的绵羊进行灌药。灌药后4小时、8小时、16小时和24小时，处死绵羊，取出吸虫，清洗后迅速在液氮中冷冻。测定发酵途径中关键代谢物的含量，并与未用雷复尼特处理宿主的对照肝片形吸虫中的含量进行比较。雷复尼特降低了糖原、苹果酸、NADH和ATP的水平。在雷复尼特处理后的前8至16小时，该途径中其他代谢物的水平有所增加。ATP和糖原的显著降低，以及总[NAD+]/[NADH]和[草酰乙酸]/[苹果酸]的增加，连同其他代谢物含量的变化，得出结论：雷复尼特在体内对肝片形吸虫的作用方式是通过解偶联氧化磷酸化。
