乙型肝炎中肿瘤坏死因子α突变前后的功能研究
Study on the Function of Tumor Necrosis Factor α Before and After Mutation in Hepatitis B.
作者信息
Zhao Zhijun, Chen Jingtao, Yin Xiaoxiang, Song Huayong, Wang Xinchun
出版信息
Hepatogastroenterology. 2015 Jun;62(140):997-1001.
BACKGROUND/AIMS: To explore the cause of functional changes of TNF-α in removing hepatitis B virus (HBV) through the structural changes of each site in TNF-α before and after mutation.
METHODOLOGY
Three typical mutant sites (TNF-α-308G/A, 857C/T and 863C/A of TNF-α) were chosen and methods like ab initio modeling was adopted for 3D modeling of TNF-α before and after mutation. The structural changes were also analyzed. Then structural changes and functional changes were brought together for comparison to infer the root cause of the changes.
RESULTS
Mutation of TNF-α-308G/A led to the production of multiple helical structures and that of 863C/A caused the production of one helical structure in its adjacent region. Mutation of 857C/T, however, did not cause the change in the basic structure of TNF-α.
CONCLUSIONS
The helical structure of TNF-α may have a positive effect on the removal of hepatitis B virus.
背景/目的：通过肿瘤坏死因子-α（TNF-α）突变前后各位点的结构变化，探讨其在清除乙型肝炎病毒（HBV）过程中功能变化的原因。
方法
选取三个典型突变位点（TNF-α的-308G/A、857C/T和863C/A），采用从头建模等方法对TNF-α突变前后进行三维建模，并分析其结构变化。然后将结构变化与功能变化进行综合比较，以推断变化的根本原因。
结果
TNF-α-308G/A突变导致多个螺旋结构产生，863C/A突变使其相邻区域产生一个螺旋结构，而857C/T突变未引起TNF-α基本结构的改变。
结论
TNF-α的螺旋结构可能对乙型肝炎病毒的清除具有积极作用。
Zotero 插件