Andresen Max, Wong Kwok-Yin, Leung Yun-Chung, Wong Wai-Ting, Chan Pak-Ho, Andresen-Vasquez Max, Alegria Leyla, Silva Camila, Tapia Pablo, Downey Patricio, Soto Dagoberto
Departamento de Medicina Intensiva, Facultad de Medicina y Hospital Clínico, Pontificia Universidad Católica de Chile, Santiago, Chile.
State Key Laboratory of Chirosciences, Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong.
Biomed Res Int. 2016;2016:4307987. doi: 10.1155/2016/4307987. Epub 2016 Jan 24.
Recently, Wong et al. have successfully developed a fluorescent biosensor based on the PenPC β-lactamase which changes its intrinsic fluorescence in presence of β-lactam antibiotics (BLAs). Here, we studied systematically this correlation among the fluorescence change of the biosensor and the concentration of different BLAs aimed at developing a novel method for estimating the concentration of a wide range of BLAs. This method showed high precision and specificity and very low interference from clinically relevant samples. We were able to monitor the pharmacokinetics of meropenem in healthy volunteers as well as in an ill animal model too, indicating that the implemented method could be suitable for clinical practice.
最近，Wong等人成功开发了一种基于PenPCβ-内酰胺酶的荧光生物传感器，该传感器在β-内酰胺抗生素（BLA）存在时会改变其固有荧光。在此，我们系统地研究了生物传感器的荧光变化与不同BLA浓度之间的这种相关性，旨在开发一种估计多种BLA浓度的新方法。该方法显示出高精度和特异性，并且来自临床相关样本的干扰非常低。我们还能够监测健康志愿者以及患病动物模型中美罗培南的药代动力学，这表明所实施的方法可能适用于临床实践。
