Fedorowicz-Strońska Olga, Kapusta Józef, Czyż Marcin, Kaczmarek Małgorzata, Pniewski Tomasz
Institute of Plant Genetics, Polish Academy of Sciences, Strzeszyńska 34, 60-479, Poznan, Poland.
Institute of Biotechnology and Antibiotics, Starościńska 5, 02-516, Warsaw, Poland.
Plant Cell Rep. 2016 May;35(5):1209-12. doi: 10.1007/s00299-016-1944-8. Epub 2016 Feb 23.
Intramuscularly delivered plant-derived M-HBsAg was compared to S-HBsAg, and as a result elicited specific anti-preS2 antibodies and significantly higher titre of anti-HBs antibodies, together with IgG isotype profile indicating some Th1 polarisation, apart from the main Th2 response. HBV prevalence is still threatening, regardless of prevention programmes using vaccines containing S-HBsAg, supplemented by third-generation vaccines, comprising also M- and L-HBsAg. Plant expression systems offer a cost-effective production option of the antigens. Plant-derived S- and M-HBsAg, intramuscularly delivered to mice, elicited anti-HBs antibodies several times higher than high responsiveness threshold titre. M-HBsAg induced stronger response of anti-HBs and also specific anti-preS2 antibodies. IgG isotype profiles indicated mainly Th2 response, yet Th1 polarisation was also pointed out, in some larger extent for M-HBsAg. These results correspond to research on CHO-derived M-HBsAg vs. commercial vaccines based on S-HBsAg and support potency of plant-derived antigens as alternative injection vaccines.
将肌肉注射的植物源M-HBsAg与S-HBsAg进行比较，结果显示其能诱导产生特异性抗preS2抗体和显著更高滴度的抗-HBs抗体，同时IgG同种型谱表明除了主要的Th2反应外，还存在一些Th1极化现象。尽管使用含S-HBsAg的疫苗并辅以包含M-和L-HBsAg的第三代疫苗的预防计划，但HBV流行率仍然构成威胁。植物表达系统提供了一种具有成本效益的抗原生产选择。肌肉注射到小鼠体内的植物源S-和M-HBsAg诱导产生的抗-HBs抗体比高反应性阈值滴度高出数倍。M-HBsAg诱导产生更强的抗-HBs反应以及特异性抗preS2抗体。IgG同种型谱主要表明Th2反应，但也指出了Th1极化现象，在M-HBsAg的情况下更为明显。这些结果与对CHO源M-HBsAg与基于S-HBsAg的商业疫苗的研究结果一致，并支持植物源抗原作为替代注射疫苗的效力。
