Yamamoto-Furusho Jesús K, Posadas-Sánchez Rosalinda, Alvarez-León Edith, Vargas-Alarcón Gilberto
Inflammatory Bowel Disease Clinic, Department of Gastroenterology, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Mexico City, Mexico.
Department of Endocrinology, Instituto Nacional de Cardiología Ignacio Chávez, Mexico City, Mexico.
Immunol Lett. 2016 Apr;172:79-83. doi: 10.1016/j.imlet.2016.02.010. Epub 2016 Feb 18.
Ulcerative colitis (UC) is a chronic condition of unknown etiology and a polygenic disease. The interleukin 27 (IL-27) have been implicated in the pathogenesis of several autoimmune diseases including inflammatory bowel disease. Several polymorphisms of IL-27 have been associated with several types of cancer and immune disorders. The aim of the present study was to evaluate the association between IL-27 gene polymorphisms and the development of UC. Four polymorphisms of IL-27p28 gene (rs181206, rs26528, rs17855750, and rs40837) and three of the Epstein-Barr virus-induced gene 3 (EBI3) (rs428253, rs4740, and rs4905) were genotyped by 5' exonuclease TaqMan genotyping assays on an ABI Prism 7900HT Fast Real-Time PCR System in 375 Mexican patients with UC and 1599 Mexican Mestizo healthy unrelated individuals. IL-27 levels were determined in 458 healthy controls. Under recessive model adjusted by age and gender, the IL-27p28 rs17855750 polymorphism was associated with decreased risk of developing UC (OR=0.27, 95% CI: 0.06-1.13, P=0.031). On the other hand, under recessive models adjusted by age and gender, the EBI3 rs428253 (OR=0.54, 95% CI: 0.29-0.99, P=0.035), rs4740 (OR=0.60, 95% CI: 0.36-1.01, P=0.046) and rs4905 (OR=0.59, 95% CI: 0.35-1.01, P=0.043) were associated with decreased risk of developing UC. Similar levels of IL-27 were observed among the genotypes of the studied polymorphisms. IL-27 polymorphisms might play a protective role for the development of UC in the Mexican population.
溃疡性结肠炎（UC）是一种病因不明的慢性疾病，属于多基因疾病。白细胞介素27（IL-27）与包括炎症性肠病在内的多种自身免疫性疾病的发病机制有关。IL-27的几种多态性与多种类型的癌症和免疫紊乱相关。本研究的目的是评估IL-27基因多态性与UC发生之间的关联。采用5'核酸外切酶TaqMan基因分型检测法，在ABI Prism 7900HT快速实时PCR系统上，对375例墨西哥UC患者和1599例墨西哥梅斯蒂索健康无关个体进行了IL-27p28基因的4种多态性（rs181206、rs26528、rs17855750和rs40837）以及爱泼斯坦-巴尔病毒诱导基因3（EBI3）的3种多态性（rs428253、rs4740和rs4905）的基因分型。在458名健康对照中测定了IL-27水平。在按年龄和性别调整的隐性模型下，IL-27p28 rs17855750多态性与UC发生风险降低相关（OR=0.27，95%CI：0.06-1.13，P=0.031）。另一方面，在按年龄和性别调整的隐性模型下，EBI3 rs428253（OR=0.54，95%CI：0.29-0.99，P=0.035）、rs4740（OR=0.60，95%CI：0.36-1.01，P=0.046）和rs4905（OR=0.59，95%CI：0.35-1.01，P=0.043）与UC发生风险降低相关。在所研究多态性的基因型中观察到相似水平的IL-27。IL-27多态性可能对墨西哥人群中UC的发生起保护作用。
