耐热芽孢杆菌 ISTVK1 从热激污水污泥中分离出来，研究其优化去除废水污染物和胞外聚合物（EPS）的生产。
Study of optimization of wastewater contaminant removal along with extracellular polymeric substances (EPS) production by a thermotolerant Bacillus sp. ISTVK1 isolated from heat shocked sewage sludge.
机构信息
School of Environmental Sciences, Jawaharlal Nehru University, New Delhi 110067, India.
School of Environmental Sciences, Jawaharlal Nehru University, New Delhi 110067, India.
出版信息
Bioresour Technol. 2016 Aug;213:21-30. doi: 10.1016/j.biortech.2016.02.040. Epub 2016 Feb 17.
The present work involved study of wastewater contaminant removal along with EPS production by a thermotolerant bacterium Bacillus sp. ISTVK1, isolated from heat shocked sewage sludge. EPS production in basal and mineral medium containing 50% filter sterilized wastewater and 0.5% sucrose was found to be 0.83±0.12gL(-1) and 0.31±0.10gL(-1) culture, respectively. GC-MS analysis of EPS revealed the presence of β-d-glucose, α-d-galactose and β-d-arabinose. FT-IR spectrum confirmed the presence carbohydrates. Box-Behnken design was used to optimize process parameters for enhanced EPS production along with % COD reduction of wastewater. The optimised conditions when used in a 1.5L bioreactor showed EPS production of 1.67±0.06gL(-1) culture and 93.0±0.21 % COD removal.
本研究分离到一株耐热菌芽孢杆菌 ISTVK1，研究了其在基本培养基和含 50%过滤灭菌废水和 0.5%蔗糖的矿物培养基中的废水污染物去除和胞外多糖（EPS）生产情况。结果表明，在基本培养基和矿物培养基中，EPS 的产量分别为 0.83±0.12gL(-1)和 0.31±0.10gL(-1)。EPS 的 GC-MS 分析表明其含有β-d-葡萄糖、α-d-半乳糖和β-d-阿拉伯糖。FT-IR 光谱证实了碳水化合物的存在。采用 Box-Behnken 设计对工艺参数进行优化，以提高 EPS 产量和废水 COD 的去除率。在 1.5L 生物反应器中使用优化条件，EPS 的产量为 1.67±0.06gL(-1)，COD 的去除率为 93.0±0.21%。
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