College of Light Industry, Textile & Food Engineering, Sichuan University, Chengdu, 610065, China.
Key Laboratory of Leather Chemistry and Engineering, Ministry of Education, Sichuan University, Chengdu, 610065, China.
Environ Sci Pollut Res Int. 2019 Aug;26(24):24900-24910. doi: 10.1007/s11356-019-05742-6. Epub 2019 Jun 25.
Klebsiella sp. TN-10, isolated from tannery wastewater, exhibited excellent nitrification ability cultivated in media with NaCl contents ranging from 0 to 35 g/L. Analysis of antioxidative enzyme activities of TN-10 stressed by NaCl showed that the stain produced more superoxide dismutase (SOD) and catalase (CAT) to fight against salt stress conditions. Moreover, the auto-aggregation index of TN-10 decreased with the increment of NaCl contents, which is consistent with results of zeta potentials. Extinction-emission matrix (EEM) and Fourier-transform infrared (FT-IR) spectrum analyses showed that the compositions of extracellular polymeric substances (EPS) by TN-10 after NaCl shock were similar. Furthermore, proteins and polysaccharides were the main components of EPS, and the contents were greatly influenced by salinity. Then, circular dichroism (CD) spectrum was used to calculate the contents of each protein secondary structure. These results provided further insights into the knowledge of nitrification characteristics, auto-aggregation performance, and EPS compositions of TN-10 during NaCl shock.
从制革废水中分离得到的 Klebsiella sp. TN-10 在 NaCl 含量为 0 到 35 g/L 的培养基中表现出良好的硝化能力。对受到 NaCl 胁迫的 TN-10 的抗氧化酶活性进行分析，结果表明该菌株产生了更多的超氧化物歧化酶 (SOD) 和过氧化氢酶 (CAT) 来对抗盐胁迫条件。此外，随着 NaCl 含量的增加，TN-10 的自聚集指数降低，这与 ζ 电位的结果一致。衰减发射矩阵 (EEM) 和傅里叶变换红外 (FT-IR) 光谱分析表明，TN-10 在 NaCl 冲击后的胞外聚合物 (EPS) 组成相似。此外，蛋白质和多糖是 EPS 的主要成分，其含量受盐度影响很大。然后，圆二色性 (CD) 光谱用于计算每种蛋白质二级结构的含量。这些结果进一步深入了解了 TN-10 在 NaCl 冲击过程中的硝化特性、自聚集性能和 EPS 组成。
