Han Sang Duk, Sung Hyun Jung, Lee Ga Hyeon, Jun Joon-Ho, Son Miwon, Kang Myung Joo
Dong-A ST Research Institute, Pharmaceutical Product Research Laboratories, Yongin 17073, Republic of Korea.
College of Pharmacy, Dankook University, Cheonan 31116, Republic of Korea.
J Microbiol Biotechnol. 2016 May 28;26(5):953-8. doi: 10.4014/jmb.1512.12037.
Chitosan-based film-forming gel is regarded as a promising vehicle for topical delivery of antimicrobial agents to skin wounds, since it protects from microbial infection and the cationic polymer itself possesses antibacterial activity. In this study, possible synergistic interaction against common skin pathogens between the cationic polymer and tyrothricin (TRC), a cyclic polypeptide antibiotic, was investigated, by determining the concentration to inhibit 90% of bacterial isolates (MIC). The addition of the polysaccharide to TRC dramatically reduced the MIC values of TRC by 1/33 and 1/4 against both methicillin-resistant and methicillinsusceptible Staphylococcus aureus, respectively. The synergism of TRC and chitosan combination against both strains was demonstrated by the checkerboard method, with a fractional inhibitory concentration index below 0.5. Moreover, co-treatment of TRC and chitosan exhibited antibacterial activity against Pseudomonas aeruginosa, due to the antibacterial activity of chitosan, whereas TRC itself did not inhibit the gram-negative bacterial growth. These findings suggested that the use of chitosan-based film for topical delivery of TRC could be an alternative to improve TRC antimicrobial activity against strains that are abundant in skin wounds.
基于壳聚糖的成膜凝胶被认为是一种有前景的载体，可用于将抗菌剂局部递送至皮肤伤口，因为它能防止微生物感染，且阳离子聚合物本身具有抗菌活性。在本研究中，通过测定抑制90%细菌分离株生长的浓度（MIC），研究了阳离子聚合物与环肽抗生素短杆菌肽（TRC）之间对常见皮肤病原体可能存在的协同相互作用。向TRC中添加多糖分别使耐甲氧西林金黄色葡萄球菌和甲氧西林敏感金黄色葡萄球菌的TRC的MIC值大幅降低了1/33和1/4。棋盘法证明了TRC与壳聚糖组合对两种菌株均具有协同作用，其部分抑菌浓度指数低于0.5。此外，由于壳聚糖的抗菌活性，TRC与壳聚糖联合处理对铜绿假单胞菌表现出抗菌活性，而TRC本身并不抑制革兰氏阴性菌的生长。这些发现表明，使用基于壳聚糖的薄膜局部递送TRC可能是提高TRC对皮肤伤口中常见菌株抗菌活性的一种替代方法。
