Singh K
Department of Microbiology, Bhopal University, India.
Biomed Environ Sci. 1989 Dec;2(4):366-75.
The effects of inorganic and organic tin compounds and various factors affecting the toxicity of the latter to Escherichia coli were studied. In comparison to stannous chloride, tributyltin chloride (TBTC) was more inhibitory to the growth of the test organism; this may be due to its greater liposolubility and ready absorption through the bacterial membrane. The toxicity of TBTC to E. coli was modulated by various factors, such as pH, sulfate and chloride ions, and the presence of glucose, succinate, and reducing agents like cysteine and dithiothreitol (DTT). TBTC was more toxic to cells in minimal than in complex media. Similarly, this compound was more inhibitory to the growth of E. coli at acid pH than at basic pH. Succinate in contrast to glucose, when used as a carbon source, made the bacterium more sensitive to TBTC. Cysteine and DTT (both at a final concentration of 0.5 mM) partially protected the cells against the inhibitory effect of this agent.
研究了无机和有机锡化合物的影响以及各种影响后者对大肠杆菌毒性的因素。与氯化亚锡相比，三丁基氯化锡（TBTC）对受试生物的生长抑制作用更强；这可能是由于其更高的脂溶性以及易于通过细菌膜吸收。TBTC对大肠杆菌的毒性受到多种因素的调节，如pH值、硫酸根离子和氯离子，以及葡萄糖、琥珀酸盐的存在，还有半胱氨酸和二硫苏糖醇（DTT）等还原剂。TBTC在基本培养基中对细胞的毒性比对复合培养基中的更大。同样，该化合物在酸性pH条件下比在碱性pH条件下对大肠杆菌的生长抑制作用更强。与葡萄糖相反，当琥珀酸盐用作碳源时，会使细菌对TBTC更敏感。半胱氨酸和DTT（最终浓度均为0.5 mM）部分保护细胞免受该试剂的抑制作用。
