Lee Sang In, Jang Hyun June, Jeon Mi-hyang, Lee Mi Ock, Kim Jeom Sun, Jeon Ik-Soo, Byun Sung June
Animal Biotechnology Division, National Institute of Animal Science, RDA, 1500, Kongjwipatjwi-ro, Iseo-myeon, Wanju-gun, Jeollabuk-do, 565-851, Republic of Korea Department of Animal Resource and Science, Dankook University, Cheonan, Choongnam, 330-714, Republic of Korea.
College of Pharmacy, Dankook University, 119 Dandae-ro, Cheonan, Chungnam 330-714, Republic of Korea.
Poult Sci. 2016 Apr;95(4):912-9. doi: 10.3382/ps/pev361. Epub 2016 Feb 15.
Cathelicidins form a family of vertebrate-specific immune molecules with an evolutionarily conserved gene structure. We analyzed the expression patterns of cathelicidin genes (CAMP, CATH3, and CATHB1) in chicken bone marrow cells (BMCs) and chicken embryonic fibroblasts (CEFs). We found that CAMP and CATHB1 were significantly up-regulated in BMCs, whereas the expression of CATH3 did not differ significantly between BMCs and CEFs. To study the mechanism underlying the up-regulation of cathelicidin genes in BMCs, we predicted the transcription factors (TFs) that bind to the 5'-flanking regions of cathelicidin genes. CEBPA, EBF1, HES1, MSX1, and ZIC3 were up-regulated in BMCs compared to CEFs. Subsequently, when a siRNA-mediated knockdown assay was performed for MSX1, the expression of CAMP and CATHB1 was decreased in BMCs. We also showed that the transcriptional activity of the CAMP promoter was decreased by mutation of the MSX1-binding sites present within the 5'-flanking region of CAMP. These results increase our understanding of the regulatory mechanisms controlling cathelicidin genes in BMCs.
杀菌肽形成了一个具有进化上保守基因结构的脊椎动物特异性免疫分子家族。我们分析了杀菌肽基因（CAMP、CATH3和CATHB1）在鸡骨髓细胞（BMCs）和鸡胚成纤维细胞（CEFs）中的表达模式。我们发现CAMP和CATHB1在BMCs中显著上调，而CATH3在BMCs和CEFs之间的表达没有显著差异。为了研究BMCs中杀菌肽基因上调的潜在机制，我们预测了与杀菌肽基因5'侧翼区域结合的转录因子（TFs）。与CEFs相比，CEBPA、EBF1、HES1、MSX1和ZIC3在BMCs中上调。随后，当对MSX1进行小干扰RNA（siRNA）介导的敲低实验时，BMCs中CAMP和CATHB1的表达降低。我们还表明，CAMP启动子的转录活性因CAMP 5'侧翼区域内存在的MSX1结合位点的突变而降低。这些结果增进了我们对BMCs中控制杀菌肽基因的调控机制的理解。
