Rahman Nafisur, Khan Sumaiya
Department of Chemistry, Aligarh Muslim University, Aligarh-202002 (UP), India.
Department of Chemistry, Aligarh Muslim University, Aligarh-202002 (UP), India.
Spectrochim Acta A Mol Biomol Spectrosc. 2016 May 5;160:26-33. doi: 10.1016/j.saa.2016.02.009. Epub 2016 Feb 17.
Ampicillin exhibited a negative and a positive cotton effects on the circular dichroism (CD) spectra in the wavelength range of 200-280nm. Cloxacillin showed a positive cotton band peaking at 228nm. Three sensitive, precise and accurate CD spectroscopic methods have been developed for the determination of ampicillin and cloxacillin. Method A was used for the determination of ampicillin in presence of cloxacillin by measuring ellipticity at 206nm. Method B and C were employed to determine ampicillin and cloxacillin based on evaluation of ellipticity at 233nm and 228nm, respectively. Methods A, B and C showed linearity in the concentration range of 10-40μgmL(-1), 5-40μgmL(-1) ampicillin and 10-80μgmL(-1) cloxacillin, respectively. The method A was successfully applied to the determination of ampicillin in commercial dosage forms containing equivalent amount of cloxacillin.
氨苄西林在200 - 280nm波长范围内的圆二色性（CD）光谱上呈现出一个负的和一个正的科顿效应。氯唑西林在228nm处显示出一个正的科顿峰。已开发出三种灵敏、精确且准确的CD光谱法用于测定氨苄西林和氯唑西林。方法A通过在206nm处测量椭圆率来测定氯唑西林存在下的氨苄西林。方法B和C分别基于对233nm和228nm处椭圆率的评估来测定氨苄西林和氯唑西林。方法A、B和C分别在10 - 40μgmL⁻¹、5 - 40μgmL⁻¹氨苄西林和10 - 80μgmL⁻¹氯唑西林的浓度范围内呈线性。方法A已成功应用于含有等量氯唑西林的市售剂型中氨苄西林的测定。
