黏菌素增强抗生素联合用药对多重耐药肺炎克雷伯菌的杀菌活性：一种比较单一峰血清浓度下多种联合杀菌试验和时间杀菌法的体外模型
Colistin Increases the Cidal Activity of Antibiotic Combinations Against Multidrug-Resistant Klebsiella pneumoniae: An In Vitro Model Comparing Multiple Combination Bactericidal Testing at One Peak Serum Concentration and Time-Kill Method.
作者信息
Mezzatesta Maria Lina, Caio Carla, Gona Floriana, Zingali Tiziana, Salerno Iasmine, Stefani Stefania
机构信息
1 Section of Microbiology, Department of Biomedical and Biotechnological Sciences, University of Catania , Catania, Italy .
2 Department of Laboratory Medicine and Advanced Biotechnologies, Mediterranean Institute for Transplantation and Advanced Specialized Therapies (ISMETT) , Palermo, Italy .
出版信息
Microb Drug Resist. 2016 Jul;22(5):360-3. doi: 10.1089/mdr.2015.0160. Epub 2016 Feb 24.
The lack of treatment for multidrug-resistant (MDR) Enterobacteriaceae often leads to the use of double or triple antibiotic combinations to increase the option of clinical success. This study analyzes multiple combination bactericidal testing (MCBT) to screen double and triple antibiotic combinations, at standard peak serum concentration, for bactericidal activity against 21 MDR Klebsiella pneumoniae isolates. This method was compared with time-killing curves. The full bactericidal activity against all strains was obtained only by adding colistin. MCBT has a potential to become a rapid method for testing multiple antibiotic combinations for MDR microorganisms when colistin is used, providing clinicians with in vitro cidal data within 48 hr of strain isolation.
多重耐药（MDR）肠杆菌科细菌缺乏有效的治疗方法，这常常导致使用双联或三联抗生素组合，以提高临床治疗成功的几率。本研究分析了多重组合杀菌试验（MCBT），以筛选在标准血清峰值浓度下对21株多重耐药肺炎克雷伯菌具有杀菌活性的双联和三联抗生素组合。该方法与时间杀菌曲线进行了比较。仅通过添加黏菌素才能获得对所有菌株的完全杀菌活性。当使用黏菌素时，MCBT有可能成为一种快速检测多重耐药微生物多种抗生素组合的方法，可在菌株分离后48小时内为临床医生提供体外杀菌数据。
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