Li P, Wu H L, Dong B H
Department of Gynecology and Obstetrics, Qilu Hospital, Shandong University, Jinan, China.
Department of Gynecology and Obstetrics, Affiliated Hospital of Taishan Medical University, Taian, China.
Genet Mol Res. 2016 Jan 8;15(1):gmr6882. doi: 10.4238/gmr.15016882.
The increasing incidence of recurrent spontaneous abortion (RSA) severely affects women's health. The involvement of the immune system during pregnancy and its contribution to RSA draw researchers' attention. Both Toll-like receptor 4 (TLR4) and chemokine (C-C motif) ligand 2 (CCL2) have been linked to various pregnancy disorders. In this study, we quantified TLR4 and CCL2 levels in a case-control study in order to elucidate the correlation between these factors and RSA, and the potential to use them as disease markers. A total of 36 RSA patients and 36 healthy control individuals were recruited for the donation of decidual and chorionic tissues and venous blood samples. Fluorescent quantitative-polymerase chain reaction was used to measure mRNA levels and enzyme linked immunosorbent assay was used to quantify serum levels of TLR4 and CCL2. RSA patients had higher TLR4 and CCL2 mRNA levels compared to controls (P < 0.05). Serum levels of the two factors were also significantly higher in RSA patients than in the control group (P < 0.05). Positive correlations were found between serum levels and tissue mRNA levels of TLR4 and CCL2. In conclusion, both TLR4 and CCL2 were closely related to the occurrence of RSA, suggesting that serum TLR4 and CCL2 levels could be used as indices for monitoring RSA in pregnant women.
复发性自然流产（RSA）发病率的不断上升严重影响了女性健康。孕期免疫系统的参与及其对RSA的影响引起了研究人员的关注。Toll样受体4（TLR4）和趋化因子（C-C基序）配体2（CCL2）均与多种妊娠疾病有关。在本研究中，我们在一项病例对照研究中对TLR4和CCL2水平进行了量化，以阐明这些因素与RSA之间的相关性，以及将它们用作疾病标志物的可能性。共招募了36例RSA患者和36名健康对照个体，用于捐赠蜕膜和绒毛组织以及静脉血样本。采用荧光定量聚合酶链反应测量mRNA水平，采用酶联免疫吸附测定法量化血清中TLR4和CCL2的水平。与对照组相比，RSA患者的TLR4和CCL2 mRNA水平更高（P < 0.05）。RSA患者血清中这两种因子的水平也显著高于对照组（P < 0.05）。TLR4和CCL2的血清水平与组织mRNA水平之间存在正相关。总之，TLR4和CCL2均与RSA的发生密切相关，表明血清TLR4和CCL2水平可作为监测孕妇RSA的指标。
