温经汤对复发性自然流产的药理活性
The Pharmacological Activity of the Wenjing Decoction in Recurrent Spontaneous Abortion.
作者信息
Cao Linhui, Chen Hui, Huang Yanxia, Chen Liudan, Kang Mengru, Liang Junxiong
机构信息
Department of Traditional Chinese Medicine, Sun Yat-sen Memorial Hospital, SunYat-sen University, Guangzhou 510120, Guangdong, China.
Department of Obstetrics and Gynecology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou 510120, Guangdong, China.
出版信息
Evid Based Complement Alternat Med. 2021 Apr 13;2021:8861394. doi: 10.1155/2021/8861394. eCollection 2021.
BACKGROUND
Recurrent spontaneous abortion (RSA) is intractable infertility and can be ameliorated with the use of traditional Chinese medicine preparation, the Wenjing decoction. This study aimed to identify the therapeutic mechanism of Wenjing decoction on specific target proteins involved in RSA.
METHODS
Wenjing decoction contains Wuzhuyu, Danggui, Chuanxiong, Guizhi, Shengjiang, Banxia, Gancao, Ejiao, Mudanpi, Chishao, Dangshen, and Maidong. Using TCMSP and BATMAN databases, we queried for active ingredients and predicted their target proteins by BATMAN. Using the edgeR package, we analyzed the differentially expressed genes (DEGs) in the GSE121950 database between control samples and RSA ( = 3). The interaction between DEGs and the predicted target proteins was identified by the Venn diagram. Using the Cytoscape software and clusterProfiler package, enrichment analysis was conducted for the intersected target proteins. Additionally, the protein-protein interaction (PPI) network and pharmacological network were generated using the Cytoscape software.
RESULTS
In total, 31, 2, 7, 7, 5, 13, 93, 11, 29, and 21 active ingredients were identified from Wuzhuyu, Danggui, Chuanxiong, Guizhi, Shengjiang, Banxia, Gancao, Mudanpi, Chishao, and Dangshen, respectively. Additionally, 100 intersected target proteins were revealed by the Venn diagram. Moreover, 98 functional terms and 24 pathways (including C-type lectin receptor signaling pathway, chemokine signaling pathway, leukocyte transendothelial migration, fluid shear stress, and atherosclerosis, and AGE-RAGE signaling pathway in diabetic complications) were enriched. In the PPI network, 10 proteins involved in these five pathways were identified, namely, TNF- (tumor necrosis factor-), IL-10 (interleukin-10), TLR4 (Toll-like receptor 4), JUN (Jun proto-oncogene), IL-1B (interleukin-1-beta), CYBB (cytochrome b558 heavy chain gene), PTGS2 (prostaglandin-endoperoxide synthase 2), APOE (apolipoprotein E), SPI1 (salmonella pathogenicity island 1), and MPO (myeloperoxidase) which showed higher degrees.
CONCLUSION
The abovementioned genes and pathways might be involved in the pharmacological activity of Wenjing decoction in RSA.
背景
复发性自然流产（RSA）是一种难治性不孕症，使用中药制剂温经汤可使其得到改善。本研究旨在确定温经汤对RSA中特定靶蛋白的治疗机制。
方法
温经汤包含吴茱萸、当归、川芎、桂枝、生姜、半夏、甘草、阿胶、牡丹皮、赤芍、党参和麦冬。利用中药系统药理学数据库与分析平台（TCMSP）和中药系统药理学数据库（BATMAN），我们查询了活性成分，并通过BATMAN预测了它们的靶蛋白。使用edgeR软件包，我们分析了GSE121950数据库中对照样本与RSA样本（n = 3）之间的差异表达基因（DEG）。通过韦恩图确定DEG与预测靶蛋白之间的相互作用。使用Cytoscape软件和clusterProfiler软件包，对相交的靶蛋白进行富集分析。此外，使用Cytoscape软件生成蛋白质-蛋白质相互作用（PPI）网络和药理网络。
结果
分别从吴茱萸、当归、川芎、桂枝、生姜、半夏、甘草、牡丹皮、赤芍和党参中鉴定出31、2、7、7、5、13、93、11、29和21种活性成分。此外，韦恩图显示有100个相交的靶蛋白。此外，还富集了98个功能术语和24条通路（包括C型凝集素受体信号通路、趋化因子信号通路、白细胞跨内皮迁移、流体切应力与动脉粥样硬化以及糖尿病并发症中的晚期糖基化终产物-晚期糖基化终产物受体信号通路）。在PPI网络中，鉴定出参与这五条通路的10种蛋白，即肿瘤坏死因子-α（TNF-α）、白细胞介素-10（IL-10）、Toll样受体4（TLR4）、原癌基因Jun（JUN）、白细胞介素-1β（IL-1B）、细胞色素b558重链基因（CYBB）、前列腺素内过氧化物合酶2（PTGS2）、载脂蛋白E（APOE）、沙门氏菌致病岛1（SPI1）和髓过氧化物酶（MPO），它们具有较高的连接度。
结论
上述基因和通路可能参与了温经汤治疗RSA的药理活性。
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