Liu H D, Zhao Z G, Du D Z, Deng C R, Fu G
Key Laboratory of Spring Rapeseed Genetic Improvement, National Key Laboratory Breeding Base for Innovation and Utilization of Plateau Crop Germplasm, Qinghai Academy of Agricultural and Forestry Sciences, Qinghai University, Xining, Qinghai Province, China.
Genet Mol Res. 2016 Jan 8;15(1):gmr7146. doi: 10.4238/gmr.15017146.
This study aimed to reveal the genetic and epigenetic variations involved in a resynthesized Brassica napus (AACC) generated from a hybridization between a B. rapa (AA) landrace and B. alboglabra (CC). Amplified fragment length polymorphism (AFLP), methylation-sensitive amplified polymorphism, and the cDNA-AFLP technique were performed to detect changes between different generations at the genome, methylation, and transcription levels. We obtained 30 lines of resynthesized B. napus with a mean 1000-seed weight of over 7.50 g. All of the lines were self-compatible, probably because both parents were self-compatible. At the genome level, the S0 generation had the lowest frequency of variations (0.18%) and the S3 generation had the highest (6.07%). The main variation pattern was the elimination of amplified restriction fragments on the CC genome from the S0 to the S4 generations. At the methylation level, we found three loci that exhibited altered methylation patterns on the parental A genome; the variance rate was 1.35%. At the transcription level, we detected 43.77% reverse mutations and 37.56% deletion mutations that mainly occurred on the A and C genomes, respectively, in the S3 generation. Our results highlight the genetic variations that occur during the diploidization of resynthesized B. napus.
本研究旨在揭示由白菜型油菜（AA）地方品种与白芥（CC）杂交产生的人工合成甘蓝型油菜（AACC）中涉及的遗传和表观遗传变异。采用扩增片段长度多态性（AFLP）、甲基化敏感扩增多态性和cDNA-AFLP技术，在基因组、甲基化和转录水平上检测不同世代之间的变化。我们获得了30个平均千粒重超过7.50 g的人工合成甘蓝型油菜株系。所有株系均自交亲和，这可能是因为双亲均自交亲和。在基因组水平上，S0代的变异频率最低（0.18%），S3代最高（6.07%）。主要变异模式是从S0代到S4代CC基因组上扩增的限制性片段的消除。在甲基化水平上，我们发现了三个在亲本A基因组上甲基化模式发生改变的位点；变异率为1.35%。在转录水平上，我们在S3代中检测到43.77%的反向突变和37.56%的缺失突变，它们主要分别发生在A和C基因组上。我们的结果突出了人工合成甘蓝型油菜二倍体化过程中发生的遗传变异。
