Wang J H, Zhou W W, Liu B X, Man D L, Yang Z D, Liu F R, Shang H
The Laboratory Medicine of First Affiliated Hospital of China Medical University, Shenyang, Liaoning, China.
Ministry of Education Key Laboratory of Medical Cell Biology.
Genet Mol Res. 2016 Jan 22;15(1):gmr6892. doi: 10.4238/gmr.15016892.
The aim of the present study is to examine the expression level of peripheral mir-21 in multiple myeloma (MM) patients and to determine its clinical significance. MM patients (30), monoclonal gammopathy of undetermined significance (MGUS) patients (14), and normal controls (20) were recruited to determine the serum level of β2-MG, IgA and IgM, IgG, λ, κ, TP, ALB, Hb, LDH, and Ca(2+). Gene expression of mir-21 was quantified by SYBR green real-time fluorescent quantitative PCR. We found that the expression level of serum mir-21 in the MM group was significantly higher than the MGUS group and the NC group (P < 0.01). According to the ISS installment, the level of mir-21, lgG, κ, and ALB in the MM group in stage I differed from that in stages II and III. The level of IgA, β2-MG in stage III was higher as compared with stage I and II (P < 0.05 and P < 0.01).The levels of mir-21, κ, (κ+λ), IgG, (IgG + IgA + IgM), and β2-MG in MM patients were positively correlated with ALB (P < 0.01). Based on the results, miR-21 plays an important role as an oncogene. Mir-21 may be important in the occurrence, development, and disease prognosis of MM.
本研究的目的是检测多发性骨髓瘤（MM）患者外周血mir-21的表达水平，并确定其临床意义。招募了30例MM患者、14例意义未明的单克隆丙种球蛋白病（MGUS）患者和20例正常对照，以测定血清β2-MG、IgA、IgM、IgG、λ、κ、总蛋白（TP）、白蛋白（ALB）、血红蛋白（Hb）、乳酸脱氢酶（LDH）和钙离子（Ca2+）水平。采用SYBR green实时荧光定量PCR法对mir-21的基因表达进行定量分析。我们发现，MM组血清mir-21的表达水平显著高于MGUS组和正常对照组（P < 0.01）。根据国际分期系统（ISS）分期，MM患者I期组的mir-21、IgG、κ和ALB水平与II期和III期组不同。III期组的IgA、β2-MG水平高于I期和II期组（P < 0.05和P < 0.01）。MM患者的mir-21、κ、（κ+λ）、IgG、（IgG + IgA + IgM）和β2-MG水平与ALB呈正相关（P < 0.01）。基于这些结果，miR-21作为一种癌基因发挥着重要作用。Mir-21可能在MM的发生、发展及疾病预后中具有重要意义。
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